TY - JOUR
T1 - Ex vivo eye tissue culture methods for xenopus
AU - Henry, Jonathan J.
AU - Perry, Kimberly J.
AU - Hamilton, Paul W.
N1 - Publisher Copyright:
© 2019 Cold Spring Harbor Laboratory Press.
PY - 2019/4
Y1 - 2019/4
N2 - Lens regeneration can be studied in whole animals following removal of the original lens (lentectomy). However, culturing a whole animal can be impractical for assays involving small molecule inhibitors or proteins. Ex vivo eye tissue culture is an alternative approach for examining lens regeneration. The ex vivo culture system offers certain advantages when compared to the in vivo regeneration assay, as the percentage of cases showing lens differentiation can exceed that seen in whole animals. This culture system also allows for the treatment of eye tissues in small volumes, which helps ensure reproducibility and reduces the amount (and cost) of small-molecule inhibitors or exogenous proteins, etc., necessary to conduct an experiment. Additionally, different eye tissues can be combined, such as nontransgenic and transgenic tissues (e.g., eyecup and cornea) that carry reporters or inducible transgenes. This approach represents a very useful tool in the analysis of lens regeneration or for simply culturing specific eye tissues, and can be used to culture either Xenopus laevis or Xenopus tropicalis eye tissues.
AB - Lens regeneration can be studied in whole animals following removal of the original lens (lentectomy). However, culturing a whole animal can be impractical for assays involving small molecule inhibitors or proteins. Ex vivo eye tissue culture is an alternative approach for examining lens regeneration. The ex vivo culture system offers certain advantages when compared to the in vivo regeneration assay, as the percentage of cases showing lens differentiation can exceed that seen in whole animals. This culture system also allows for the treatment of eye tissues in small volumes, which helps ensure reproducibility and reduces the amount (and cost) of small-molecule inhibitors or exogenous proteins, etc., necessary to conduct an experiment. Additionally, different eye tissues can be combined, such as nontransgenic and transgenic tissues (e.g., eyecup and cornea) that carry reporters or inducible transgenes. This approach represents a very useful tool in the analysis of lens regeneration or for simply culturing specific eye tissues, and can be used to culture either Xenopus laevis or Xenopus tropicalis eye tissues.
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U2 - 10.1101/pdb.prot101535
DO - 10.1101/pdb.prot101535
M3 - Article
C2 - 29895561
AN - SCOPUS:85064190629
SN - 1940-3402
VL - 2019
SP - 274
EP - 278
JO - Cold Spring Harbor protocols
JF - Cold Spring Harbor protocols
IS - 4
ER -