Evolutionary implications of the cDNA sequence of the single lactate dehydrogenase of a lamprey

D. W. Stock, G. S. Whitt

Research output: Contribution to journalArticlepeer-review

Abstract

All vertebrates other than lampreys exhibit multiple loci encoding lactate dehydrogenase [L-LDH; (S)-lactate:NAD+ oxidoreductase, EC 1.1.1.27]. Of these loci, Ldh-A is expressed predominantly in muscle, Ldh-B is expressed predominantly in heart, and Ldh-C (where present) exhibits different tissue- restricted patterns of expression depending on the taxon. To examine the relationship of the single LDH of lampreys to other vertebrate LDHs, we have determined the cDNA sequence of the LDH of the sea lamprey Petromyzon marinus and compared it to previously published sequences from bacteria, plants, and vertebrates. The lamprey sequence exhibits a mixture of features of both LDH- A and LDH-B at the amino acid level that may account for its intermediate kinetic properties. Both distance and maximum parsimony analyses strongly reject a relationship of lamprey LDH with mammalian LDH-C but do not significantly distinguish among remaining alternative phylogenetic hypotheses. Evolutionary parsimony analyses suggest that the lamprey LDH is related to Ldh-A and that the single locus condition has arisen as a result of the loss of Ldh-B (prior to the appearance of Ldh-C). The collection of LDH sequences for further studies of the evolution of the vertebrate LDH gene family will be facilitated by the PCR approach that we have used to obtain the lamprey sequence.

Original languageEnglish (US)
Pages (from-to)1799-1803
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume89
Issue number5
DOIs
StatePublished - 1992

Keywords

  • bootstrapping
  • evolutionary parsimony
  • maximum parsimony
  • molecular phylogeny
  • polymerase chain reaction

ASJC Scopus subject areas

  • General

Fingerprint

Dive into the research topics of 'Evolutionary implications of the cDNA sequence of the single lactate dehydrogenase of a lamprey'. Together they form a unique fingerprint.

Cite this