Evolution of protein-coupled RNA dynamics during hierarchical assembly of ribosomal complexes

Sanjaya C. Abeysirigunawardena; Hajin Kim; Jonathan Lai; Kaushik Ragunathan; Mollie C. Rappé; Zaida Luthey-Schulten; Taekjip Ha; Sarah A. Woodson

doi:10.1038/s41467-017-00536-1

Evolution of protein-coupled RNA dynamics during hierarchical assembly of ribosomal complexes

Sanjaya C. Abeysirigunawardena
, Hajin Kim
, Jonathan Lai
, Kaushik Ragunathan
, Mollie C. Rappé
, Zaida Luthey-Schulten
, Taekjip Ha
, Sarah A. Woodson

Research output: Contribution to journal › Article › peer-review

Abstract

Ribosomes assemble through the hierarchical addition of proteins to a ribosomal RNA scaffold. Here the authors use three-color single-molecule FRET to show how the dynamics of the rRNA dictate the order in which multiple proteins assemble on the 5′ domain of the E. coli 16S rRNA.

Original language	English (US)
Article number	492
Journal	Nature communications
Volume	8
Issue number	1
DOIs	https://doi.org/10.1038/s41467-017-00536-1
State	Published - Dec 1 2017

ASJC Scopus subject areas

General Chemistry
General Biochemistry, Genetics and Molecular Biology
General Physics and Astronomy

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10.1038/s41467-017-00536-1

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title = "Evolution of protein-coupled RNA dynamics during hierarchical assembly of ribosomal complexes",

abstract = "Ribosomes assemble through the hierarchical addition of proteins to a ribosomal RNA scaffold. Here the authors use three-color single-molecule FRET to show how the dynamics of the rRNA dictate the order in which multiple proteins assemble on the 5′ domain of the E. coli 16S rRNA.",

author = "Abeysirigunawardena, \{Sanjaya C.\} and Hajin Kim and Jonathan Lai and Kaushik Ragunathan and Rapp{\'e}, \{Mollie C.\} and Zaida Luthey-Schulten and Taekjip Ha and Woodson, \{Sarah A.\}",

note = "We would like to thank G. Culver for the gift of plasmids. This work was supported by the National Institutes of Health (R01 GM68019 to S.A.W. and R35 GM122569 to T.H.), the Howard Hughes Medical Institute (T.H.), and the National Science Foundation (MCB 12-44570 to Z.L.-S. and PHY 1430124 to Z.L.-S. and T.H.). H.K. was supported by the National Research Foundation of Korea (2014R1A1A1003949), IBS-R022-D1, and UNIST research fund (1.170009.01). J.L. was supported by the US Department of Energy, Office of Science, Biological and Environmental Research as part of the Adaptive Bio-systems Imaging Scientific Focus Area.",

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doi = "10.1038/s41467-017-00536-1",

language = "English (US)",

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TY - JOUR

T1 - Evolution of protein-coupled RNA dynamics during hierarchical assembly of ribosomal complexes

AU - Abeysirigunawardena, Sanjaya C.

AU - Kim, Hajin

AU - Lai, Jonathan

AU - Ragunathan, Kaushik

AU - Rappé, Mollie C.

AU - Luthey-Schulten, Zaida

AU - Ha, Taekjip

AU - Woodson, Sarah A.

N1 - We would like to thank G. Culver for the gift of plasmids. This work was supported by the National Institutes of Health (R01 GM68019 to S.A.W. and R35 GM122569 to T.H.), the Howard Hughes Medical Institute (T.H.), and the National Science Foundation (MCB 12-44570 to Z.L.-S. and PHY 1430124 to Z.L.-S. and T.H.). H.K. was supported by the National Research Foundation of Korea (2014R1A1A1003949), IBS-R022-D1, and UNIST research fund (1.170009.01). J.L. was supported by the US Department of Energy, Office of Science, Biological and Environmental Research as part of the Adaptive Bio-systems Imaging Scientific Focus Area.

PY - 2017/12/1

Y1 - 2017/12/1

N2 - Ribosomes assemble through the hierarchical addition of proteins to a ribosomal RNA scaffold. Here the authors use three-color single-molecule FRET to show how the dynamics of the rRNA dictate the order in which multiple proteins assemble on the 5′ domain of the E. coli 16S rRNA.

AB - Ribosomes assemble through the hierarchical addition of proteins to a ribosomal RNA scaffold. Here the authors use three-color single-molecule FRET to show how the dynamics of the rRNA dictate the order in which multiple proteins assemble on the 5′ domain of the E. coli 16S rRNA.

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UR - https://www.scopus.com/pages/publications/85028972421#tab=citedBy

U2 - 10.1038/s41467-017-00536-1

DO - 10.1038/s41467-017-00536-1

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AN - SCOPUS:85028972421

SN - 2041-1723

VL - 8

JO - Nature communications

JF - Nature communications

IS - 1

M1 - 492

ER -

Evolution of protein-coupled RNA dynamics during hierarchical assembly of ribosomal complexes

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