Evolution of eukaryotic transcription: Insights from the genome of Giardia lamblia

Aaron A. Best, Hilary G. Morrison, Andrew G. McArthur, Mitchell L. Sogin, Gary J. Olsen

Research output: Contribution to journalArticle

Abstract

The Giardia lamblia genome sequencing project affords us a unique opportunity to conduct comparative analyses of core cellular systems between early and late-diverging eukaryotes on a genome-wide scale. We report a survey to identify canonical transcription components in Giardia, focusing on RNA polymerase (RNAP) subunits and transcription-initiation factors. Our survey revealed that Giardia contains homologs to 21 of the 28 polypeptides comprising eukaryal RNAPI, RNAPII, and RNAPIII; six of the seven RNAP subunits without giardial homologs are polymerase specific. Components of only four of the 12 general transcription initiation factors have giardial homologs. Surprisingly, giardial TATA-binding protein (TBP) is highly divergent with respect to archaeal and higher eukaryotic TBPs, and a giardial homolog of transcription factor IIB was not identified. We conclude that Giardia represents a transition during the evolution of eukaryal transcription systems, exhibiting a relatively complete set of RNAP subunits and a rudimentary basal initiation apparatus for each transcription system. Most class-specific RNAP subunits and basal initiation factors appear to have evolved after the divergence of Giardia from the main eukaryotic line of descent. Consequently, Giardia is predicted to be unique in many aspects of transcription initiation with respect to paradigms derived from studies in crown eukaryotes.

Original languageEnglish (US)
Pages (from-to)1537-1547
Number of pages11
JournalGenome Research
Volume14
Issue number8
DOIs
StatePublished - Aug 1 2004

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Giardia
Giardia lamblia
DNA-Directed RNA Polymerases
Peptide Initiation Factors
Genome
Eukaryota
Transcription Factor TFIIB
General Transcription Factors
TATA-Box Binding Protein
Crowns
Transcription Factors
Peptides

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

Cite this

Evolution of eukaryotic transcription : Insights from the genome of Giardia lamblia. / Best, Aaron A.; Morrison, Hilary G.; McArthur, Andrew G.; Sogin, Mitchell L.; Olsen, Gary J.

In: Genome Research, Vol. 14, No. 8, 01.08.2004, p. 1537-1547.

Research output: Contribution to journalArticle

Best, Aaron A. ; Morrison, Hilary G. ; McArthur, Andrew G. ; Sogin, Mitchell L. ; Olsen, Gary J. / Evolution of eukaryotic transcription : Insights from the genome of Giardia lamblia. In: Genome Research. 2004 ; Vol. 14, No. 8. pp. 1537-1547.
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