TY - JOUR
T1 - Evidence that the bypassing ribosome travels through the coding gap
AU - Gallant, Jonathan
AU - Bonthuis, Paul
AU - Lindsley, Dale
PY - 2003/11/11
Y1 - 2003/11/11
N2 - In translational bypassing, a peptidyl-tRNA::ribosome complex skips over a number of nucleotides in a messenger sequence and resumes protein chain elongation after a "landing site" down-stream of the bypassed region. The present experiments demonstrate that the complex "scans" processively through the bypassed region. This conclusion rests on three observations. (i) When two potential "landing sites" are present, the protein sequence of the product shows that virtually all ribosomes land at the first and virtually none at the second. (ii) In such a sequence with two landing sites, the presence of a terminator triplet in phase in the coding region immediately after the first landing site drastically reduces the efficiency of bypassing. (iii) Internally complementary sequences that can form a stable stem/loop in the bypassed region significantly reduce the efficiency of bypassing. We analyze by-passing from a given "takeoff" site to "landing sites" at different distances downstream so as to derive estimates of the frequency of ribosome takeoff and of the stability of the bypassing complex.
AB - In translational bypassing, a peptidyl-tRNA::ribosome complex skips over a number of nucleotides in a messenger sequence and resumes protein chain elongation after a "landing site" down-stream of the bypassed region. The present experiments demonstrate that the complex "scans" processively through the bypassed region. This conclusion rests on three observations. (i) When two potential "landing sites" are present, the protein sequence of the product shows that virtually all ribosomes land at the first and virtually none at the second. (ii) In such a sequence with two landing sites, the presence of a terminator triplet in phase in the coding region immediately after the first landing site drastically reduces the efficiency of bypassing. (iii) Internally complementary sequences that can form a stable stem/loop in the bypassed region significantly reduce the efficiency of bypassing. We analyze by-passing from a given "takeoff" site to "landing sites" at different distances downstream so as to derive estimates of the frequency of ribosome takeoff and of the stability of the bypassing complex.
UR - http://www.scopus.com/inward/record.url?scp=0345255236&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0345255236&partnerID=8YFLogxK
U2 - 10.1073/pnas.2233745100
DO - 10.1073/pnas.2233745100
M3 - Article
C2 - 14576279
AN - SCOPUS:0345255236
SN - 0027-8424
VL - 100
SP - 13430
EP - 13435
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 23
ER -