Evidence that rabbit cytochrome P-450 k is encoded by the plasmid pP-450 PBc2

Malcolm J. Finlayson, Byron Kemper, Nancy Browne, Eric F. Johnson

Research output: Contribution to journalArticlepeer-review


The complete coding sequence for P-450 PBc2 was inserted into a T7-phage promoter system, and a capped cRNA was generated using T7 RNA polymerase. The P-450 PBc2 cRNA was translated in a rabbit reticulocyte lysate. The in vitro translation product was indirectly immunoprecipitated by the monoclonal antibodies 2F5 and 3C3 that recognize P-450 K. SDSpolyacrylamide gel electrophoresis revealed that the translated protein product exhibits the same relative electrophoretic mobility as P-450 K. The N-terminal amino acid sequence was determined to be MDLVVVLGL-LS-LLLLSL- for P-450 K immunopurified from rabbit kidney using the monoclonal antibody 2F5. This sequence agrees with the predicted amino acid sequence derived from the P-450 PBc2 cDNA. These results indicate that P-450 K or a protein closely related to P-450 K is encoded by the plasmid pP-450 PBc2.

Original languageEnglish (US)
Pages (from-to)728-733
Number of pages6
JournalBiochemical and Biophysical Research Communications
Issue number2
StatePublished - Dec 15 1986
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology


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