Evidence for distinct electron transfer processes in terminal oxidases from different origin by means of protein film voltammetry

Thomas Meyer, Frédéric Melin, Hao Xie, Iris Von Der Hocht, Sylvia K. Choi, Mohamed R. Noor, Hartmut Michel, Robert B. Gennis, Tewfik Soulimane, Petra Hellwig

Research output: Contribution to journalArticle

Abstract

Cytochrome aa3 from Paracoccus denitrificans and cytochrome ba3 from Thermus thermophilus, two distinct members of the heme-copper oxidase superfamily, were immobilized on electrodes modified with gold nanoparticles. This procedure allowed us to achieve direct electron transfer between the enzyme and the gold nanoparticles and to obtain evidence for different electrocatalytic properties of the two enzymes. The pH dependence and thermostability reveal that the enzymes are highly adapted to their native environments. These results suggest that evolution resulted in different solutions to the common problem of electron transfer to oxygen.

Original languageEnglish (US)
Pages (from-to)10854-10857
Number of pages4
JournalJournal of the American Chemical Society
Volume136
Issue number31
DOIs
StatePublished - Aug 6 2014

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Voltammetry
Oxidoreductases
Enzymes
Electrons
enzyme
Proteins
Gold
electron
Nanoparticles
protein
cytochrome
gold
Paracoccus denitrificans
Thermus thermophilus
Electron Transport Complex IV
Heme
Electrodes
electrode
Oxygen
copper

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

Cite this

Evidence for distinct electron transfer processes in terminal oxidases from different origin by means of protein film voltammetry. / Meyer, Thomas; Melin, Frédéric; Xie, Hao; Von Der Hocht, Iris; Choi, Sylvia K.; Noor, Mohamed R.; Michel, Hartmut; Gennis, Robert B.; Soulimane, Tewfik; Hellwig, Petra.

In: Journal of the American Chemical Society, Vol. 136, No. 31, 06.08.2014, p. 10854-10857.

Research output: Contribution to journalArticle

Meyer, T, Melin, F, Xie, H, Von Der Hocht, I, Choi, SK, Noor, MR, Michel, H, Gennis, RB, Soulimane, T & Hellwig, P 2014, 'Evidence for distinct electron transfer processes in terminal oxidases from different origin by means of protein film voltammetry', Journal of the American Chemical Society, vol. 136, no. 31, pp. 10854-10857. https://doi.org/10.1021/ja505126v
Meyer, Thomas ; Melin, Frédéric ; Xie, Hao ; Von Der Hocht, Iris ; Choi, Sylvia K. ; Noor, Mohamed R. ; Michel, Hartmut ; Gennis, Robert B. ; Soulimane, Tewfik ; Hellwig, Petra. / Evidence for distinct electron transfer processes in terminal oxidases from different origin by means of protein film voltammetry. In: Journal of the American Chemical Society. 2014 ; Vol. 136, No. 31. pp. 10854-10857.
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