Evidence for a 13,14-Cis Cycle in Bacteriorhodopsin

Paul Tavan; Klaus Schulten

doi:10.1016/S0006-3495(86)83441-5

Evidence for a 13,14-Cis Cycle in Bacteriorhodopsin

Paul Tavan, Klaus Schulten

Research output: Contribution to journal › Article › peer-review

Abstract

We discuss to what extent the vibrational spectra of bacteriorhodopsin that have been observed and assigned by Smith et al. (1, 2) by means of resonance Raman and by Gerwert and Siebert (EMBO (Eur. Mol. Biol. Organ.) J. In press) by means of infrared absorption experiments are in agreement with a photo-cycle of bacteriorhodopsin that involves the sequence BR, IO(all-trans) → K(13,14-cis) → L(13,14-cis) → M(13-cis) → N(13-cis) → O(all-trans). Our discussion is based on a quantumchemical modified neglect of diatomic overlap [MNDO] calculation of the vibrational spectra of the relevant isomers of the protonated retinal Schiff base. In particular, we investigated in these calculations the effects of different charge environments on the frequencies of the relevant C-C single bond stretching vibrations of these isomers.

Original language	English (US)
Pages (from-to)	81-89
Number of pages	9
Journal	Biophysical journal
Volume	50
Issue number	1
DOIs	https://doi.org/10.1016/S0006-3495(86)83441-5
State	Published - 1986
Externally published	Yes

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Biophysics

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10.1016/S0006-3495(86)83441-5

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title = "Evidence for a 13,14-Cis Cycle in Bacteriorhodopsin",

abstract = "We discuss to what extent the vibrational spectra of bacteriorhodopsin that have been observed and assigned by Smith et al. (1, 2) by means of resonance Raman and by Gerwert and Siebert (EMBO (Eur. Mol. Biol. Organ.) J. In press) by means of infrared absorption experiments are in agreement with a photo-cycle of bacteriorhodopsin that involves the sequence BR, IO(all-trans) → K(13,14-cis) → L(13,14-cis) → M(13-cis) → N(13-cis) → O(all-trans). Our discussion is based on a quantumchemical modified neglect of diatomic overlap [MNDO] calculation of the vibrational spectra of the relevant isomers of the protonated retinal Schiff base. In particular, we investigated in these calculations the effects of different charge environments on the frequencies of the relevant C-C single bond stretching vibrations of these isomers.",

author = "Paul Tavan and Klaus Schulten",

note = "Funding Information: This project has been supported by the Deutsche Forschungsgemein- schaft (SFB-143 C1).",

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doi = "10.1016/S0006-3495(86)83441-5",

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T1 - Evidence for a 13,14-Cis Cycle in Bacteriorhodopsin

AU - Tavan, Paul

AU - Schulten, Klaus

N1 - Funding Information: This project has been supported by the Deutsche Forschungsgemein- schaft (SFB-143 C1).

PY - 1986

Y1 - 1986

N2 - We discuss to what extent the vibrational spectra of bacteriorhodopsin that have been observed and assigned by Smith et al. (1, 2) by means of resonance Raman and by Gerwert and Siebert (EMBO (Eur. Mol. Biol. Organ.) J. In press) by means of infrared absorption experiments are in agreement with a photo-cycle of bacteriorhodopsin that involves the sequence BR, IO(all-trans) → K(13,14-cis) → L(13,14-cis) → M(13-cis) → N(13-cis) → O(all-trans). Our discussion is based on a quantumchemical modified neglect of diatomic overlap [MNDO] calculation of the vibrational spectra of the relevant isomers of the protonated retinal Schiff base. In particular, we investigated in these calculations the effects of different charge environments on the frequencies of the relevant C-C single bond stretching vibrations of these isomers.

AB - We discuss to what extent the vibrational spectra of bacteriorhodopsin that have been observed and assigned by Smith et al. (1, 2) by means of resonance Raman and by Gerwert and Siebert (EMBO (Eur. Mol. Biol. Organ.) J. In press) by means of infrared absorption experiments are in agreement with a photo-cycle of bacteriorhodopsin that involves the sequence BR, IO(all-trans) → K(13,14-cis) → L(13,14-cis) → M(13-cis) → N(13-cis) → O(all-trans). Our discussion is based on a quantumchemical modified neglect of diatomic overlap [MNDO] calculation of the vibrational spectra of the relevant isomers of the protonated retinal Schiff base. In particular, we investigated in these calculations the effects of different charge environments on the frequencies of the relevant C-C single bond stretching vibrations of these isomers.

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Evidence for a 13,14-Cis Cycle in Bacteriorhodopsin

Abstract

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