Evaluation of the N2-fixation capacity of four soybean genotypes by several methods

R. D. Guffy, R. M. Vanden Heuvel, B. L. Vasilas, Randall L Nelson, M. A. Frobish, J. D. Hesketh

Research output: Contribution to journalArticle

Abstract

Plant-N derived from N2-fixation (N2-fixed) and the percentage of plant-N derived from N2-fixation (% Ndff) were determined for four field-grown soybean lines by several techniques. The objective was to determined which technique would distinguish between the lines on the basis of their N2-fixing abilities. Variables in the techniques included method (total N or 15N isotope dilution), plant sample (seed, shoot at physiological maturity and whole plant) and placement of control (non-nodulating soybean) plots. Estimates of N2-fixed generated by isotope dilution were. on average, higher than those generated by the difference method (total N). Greater distinction between the genotypes was possible using the difference method than with isotope dilution. Separation of genotypes on the basis of N2-fixed as estimated by isotope dilution was not affected by the tissue sample used (seed, shoot or whole plant).

Original languageEnglish (US)
Pages (from-to)339-342
Number of pages4
JournalSoil Biology and Biochemistry
Volume21
Issue number3
DOIs
StatePublished - 1989

Fingerprint

Nitrogen Fixation
Soybeans
fixation
soybean
genotype
Isotopes
isotope dilution technique
Genotype
soybeans
dilution
isotope
Seeds
shoot
seed
methodology
shoots
seeds
evaluation
method
sampling

ASJC Scopus subject areas

  • Microbiology
  • Soil Science

Cite this

Guffy, R. D., Vanden Heuvel, R. M., Vasilas, B. L., Nelson, R. L., Frobish, M. A., & Hesketh, J. D. (1989). Evaluation of the N2-fixation capacity of four soybean genotypes by several methods. Soil Biology and Biochemistry, 21(3), 339-342. https://doi.org/10.1016/0038-0717(89)90140-5

Evaluation of the N2-fixation capacity of four soybean genotypes by several methods. / Guffy, R. D.; Vanden Heuvel, R. M.; Vasilas, B. L.; Nelson, Randall L; Frobish, M. A.; Hesketh, J. D.

In: Soil Biology and Biochemistry, Vol. 21, No. 3, 1989, p. 339-342.

Research output: Contribution to journalArticle

Guffy, RD, Vanden Heuvel, RM, Vasilas, BL, Nelson, RL, Frobish, MA & Hesketh, JD 1989, 'Evaluation of the N2-fixation capacity of four soybean genotypes by several methods', Soil Biology and Biochemistry, vol. 21, no. 3, pp. 339-342. https://doi.org/10.1016/0038-0717(89)90140-5
Guffy, R. D. ; Vanden Heuvel, R. M. ; Vasilas, B. L. ; Nelson, Randall L ; Frobish, M. A. ; Hesketh, J. D. / Evaluation of the N2-fixation capacity of four soybean genotypes by several methods. In: Soil Biology and Biochemistry. 1989 ; Vol. 21, No. 3. pp. 339-342.
@article{9c18603666414d33ac6522aaf663efa1,
title = "Evaluation of the N2-fixation capacity of four soybean genotypes by several methods",
abstract = "Plant-N derived from N2-fixation (N2-fixed) and the percentage of plant-N derived from N2-fixation ({\%} Ndff) were determined for four field-grown soybean lines by several techniques. The objective was to determined which technique would distinguish between the lines on the basis of their N2-fixing abilities. Variables in the techniques included method (total N or 15N isotope dilution), plant sample (seed, shoot at physiological maturity and whole plant) and placement of control (non-nodulating soybean) plots. Estimates of N2-fixed generated by isotope dilution were. on average, higher than those generated by the difference method (total N). Greater distinction between the genotypes was possible using the difference method than with isotope dilution. Separation of genotypes on the basis of N2-fixed as estimated by isotope dilution was not affected by the tissue sample used (seed, shoot or whole plant).",
author = "Guffy, {R. D.} and {Vanden Heuvel}, {R. M.} and Vasilas, {B. L.} and Nelson, {Randall L} and Frobish, {M. A.} and Hesketh, {J. D.}",
year = "1989",
doi = "10.1016/0038-0717(89)90140-5",
language = "English (US)",
volume = "21",
pages = "339--342",
journal = "Soil Biology and Biochemistry",
issn = "0038-0717",
publisher = "Elsevier Limited",
number = "3",

}

TY - JOUR

T1 - Evaluation of the N2-fixation capacity of four soybean genotypes by several methods

AU - Guffy, R. D.

AU - Vanden Heuvel, R. M.

AU - Vasilas, B. L.

AU - Nelson, Randall L

AU - Frobish, M. A.

AU - Hesketh, J. D.

PY - 1989

Y1 - 1989

N2 - Plant-N derived from N2-fixation (N2-fixed) and the percentage of plant-N derived from N2-fixation (% Ndff) were determined for four field-grown soybean lines by several techniques. The objective was to determined which technique would distinguish between the lines on the basis of their N2-fixing abilities. Variables in the techniques included method (total N or 15N isotope dilution), plant sample (seed, shoot at physiological maturity and whole plant) and placement of control (non-nodulating soybean) plots. Estimates of N2-fixed generated by isotope dilution were. on average, higher than those generated by the difference method (total N). Greater distinction between the genotypes was possible using the difference method than with isotope dilution. Separation of genotypes on the basis of N2-fixed as estimated by isotope dilution was not affected by the tissue sample used (seed, shoot or whole plant).

AB - Plant-N derived from N2-fixation (N2-fixed) and the percentage of plant-N derived from N2-fixation (% Ndff) were determined for four field-grown soybean lines by several techniques. The objective was to determined which technique would distinguish between the lines on the basis of their N2-fixing abilities. Variables in the techniques included method (total N or 15N isotope dilution), plant sample (seed, shoot at physiological maturity and whole plant) and placement of control (non-nodulating soybean) plots. Estimates of N2-fixed generated by isotope dilution were. on average, higher than those generated by the difference method (total N). Greater distinction between the genotypes was possible using the difference method than with isotope dilution. Separation of genotypes on the basis of N2-fixed as estimated by isotope dilution was not affected by the tissue sample used (seed, shoot or whole plant).

UR - http://www.scopus.com/inward/record.url?scp=0000631633&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0000631633&partnerID=8YFLogxK

U2 - 10.1016/0038-0717(89)90140-5

DO - 10.1016/0038-0717(89)90140-5

M3 - Article

AN - SCOPUS:0000631633

VL - 21

SP - 339

EP - 342

JO - Soil Biology and Biochemistry

JF - Soil Biology and Biochemistry

SN - 0038-0717

IS - 3

ER -