Evaluation of the hypoglycemic potential of a black bean hydrolyzed protein isolate and its pure peptides using in silico, in vitro and in vivo approaches

The objective was to evaluate the in silico, in vitro and in vivo effects of a black bean hydrolyzed protein isolate (HPI) and its pure peptides on glucose absorption. By interacting with their protein motif, pure peptides blocked glucose transporters GLUT2 and SGLT1. HPI (10 mg/mL) reduced formation of intracellular oxygen reactive species by 71%. Glucose absorption decreased 21.5% after 24 h treatment when using the Caco-2 cell model. Oral glucose tolerance test in rats showed a 24.5% decrease on postprandial glucose (p < 0.05) (50 mg HPI /kg BW). In the hyperglycemic rat model, HPI caused a reduction of blood glucose, in a dose-dependent manner. The lowest fasting glucose was found in rats receiving 150 and 200 mg/kg BW/day HPI, (p < 0.05). The black bean HPI is an inexpensive food source of bioactive compounds that could be used in the management of blood glucose.