Evaluation of plasma fibrinogen concentration as a diagnostic indicator of inflammation in red-eared sliders (Trachemys scripta elegans)

A. Russell Moore, Matthew C. Allender, Mark A. Mitchell, Amy L. MacNeill

Research output: Contribution to journalArticlepeer-review



Design—Prospective induced-disease model and prospective cross-sectional study. Sample—Plasma samples from 12 purpose-bred red-eared sliders and 153 farm-raised redeared sliders.

Procedures—A modification of the Jacobsson method was developed to measure fibrinogen concentration in platelet-poor plasma from red-eared sliders. Purpose-bred turtles had been inoculated with a ranavirus (n = 4) or sterile PBS solution (8) as part of another study. Farm-raised red-eared sliders were categorized as healthy (n = 138) or overtly ill (15) on the basis of physical examination findings at the time of blood sample collection. Samples from 124 of the 138 healthy red-eared sliders were used to establish a fibrinogen concentration reference interval as measured by the modified Jacobsson method. Fibrinogen concentrations in ranavirus-infected and physically ill turtles were compared with those of healthy turtles to determine whether fibrinogen concentration would be a useful diagnostic indicator of inflammation in red-eared sliders.

Results—The modified Jacobsson method was reliably used to measure fibrinogen concentration. The fibrinogen concentration reference interval from healthy reproductively active female red-eared sliders was right skewed. Fibrinogen concentration did not differ significantly between healthy red-eared sliders and ranavirus-infected or overtly ill red-eared sliders.

Conclusions and Clinical Relevance—A reference interval for red-eared slider plasma fibrinogen concentration was established and partitioned by sex to account for considerable right skewing observed for females.

Original languageEnglish (US)
Pages (from-to)245-253
Number of pages9
JournalJournal of the American Veterinary Medical Association
Issue number2
StatePublished - Jan 15 2015

ASJC Scopus subject areas

  • veterinary(all)


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