Objective—To critically evaluate plasma ﬁbrinogen concentration as a diagnostic indicator of inﬂammation in red-eared sliders (Trachemys scripta elegans)
Design—Prospective induced-disease model and prospective cross-sectional study. Sample—Plasma samples from 12 purpose-bred red-eared sliders and 153 farm-raised redeared sliders.
Procedures—A modiﬁcation of the Jacobsson method was developed to measure ﬁbrinogen concentration in platelet-poor plasma from red-eared sliders. Purpose-bred turtles had been inoculated with a ranavirus (n = 4) or sterile PBS solution (8) as part of another study. Farm-raised red-eared sliders were categorized as healthy (n = 138) or overtly ill (15) on the basis of physical examination ﬁndings at the time of blood sample collection. Samples from 124 of the 138 healthy red-eared sliders were used to establish a ﬁbrinogen concentration reference interval as measured by the modiﬁed Jacobsson method. Fibrinogen concentrations in ranavirus-infected and physically ill turtles were compared with those of healthy turtles to determine whether ﬁbrinogen concentration would be a useful diagnostic indicator of inﬂammation in red-eared sliders.
Results—The modiﬁed Jacobsson method was reliably used to measure ﬁbrinogen concentration. The ﬁbrinogen concentration reference interval from healthy reproductively active female red-eared sliders was right skewed. Fibrinogen concentration did not differ signiﬁcantly between healthy red-eared sliders and ranavirus-infected or overtly ill red-eared sliders.
Conclusions and Clinical Relevance—A reference interval for red-eared slider plasma ﬁbrinogen concentration was established and partitioned by sex to account for considerable right skewing observed for females. Fibrinogen concentration was not a useful indicator of inﬂammation in red-eared sliders with ranavirus infection or other overt illnesses
|Original language||English (US)|
|Number of pages||9|
|Journal||Journal of the American Veterinary Medical Association|
|State||Published - Jan 15 2015|
ASJC Scopus subject areas