Evaluation of ethanol production activity by engineered saccharomyces cerevisiae fermenting cellobiose through the phosphorolytic pathway in simultaneous saccharification and fermentation of cellulose

Won Heong Lee, Yong Su Jin

Research output: Contribution to journalArticlepeer-review

Abstract

In simultaneous saccharification and fermentation (SSF) for production of cellulosic biofuels, engineered Saccharomyces cerevisiae capable of fermenting cellobiose has provided several benefits, such as lower enzyme costs and faster fermentation rate compared with wild-type S. cerevisiae fermenting glucose. In this study, the effects of an alternative intracellular cellobiose utilization pathway—a phosphorolytic pathway based on a mutant cellodextrin transporter (CDT-1 (F213L)) and cellobiose phosphorylase (SdCBP)—was investigated by comparing with a hydrolytic pathway based on the same transporter and an intracellular β-glucosidase (GH1-1) for their SSF performances under various conditions. Whereas the phosphorolytic and hydrolytic cellobiose-fermenting S. cerevisiae strains performed similarly under the anoxic SSF conditions, the hydrolytic S. cerevisiae performed slightly better than the phosphorolytic S. cerevisiae under the microaerobic SSF conditions. Nonetheless, the phosphorolytic S. cerevisiae expressing the mutant CDT-1 showed better ethanol production than the glucose-fermenting S. cerevisiae with an extracellular β-glucosidase, regardless of SSF conditions. These results clearly prove that introduction of the intracellular cellobiose metabolic pathway into yeast can be effective on cellulosic ethanol production in SSF. They also demonstrate that enhancement of cellobiose transport activity in engineered yeast is the most important factor affecting the efficiency of SSF of cellulose.

Original languageEnglish (US)
Pages (from-to)1649-1656
Number of pages8
JournalJournal of Microbiology and Biotechnology
Volume27
Issue number9
DOIs
StatePublished - Sep 28 2017

Keywords

  • Cellobiose phosphorylase
  • Cellobiose transporter
  • Cellulosic ethanol
  • Engineered saccharomyces cerevisiae
  • Simultaneous saccharification and fermentation

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

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