TY - JOUR
T1 - Evaluation of circulating leukocyte transcriptome and its relationship with immune function and blood markers in dairy cows during the transition period
AU - Minuti, Andrea
AU - Jahan, Nusrat
AU - Lopreiato, Vincenzo
AU - Piccioli-Cappelli, Fiorenzo
AU - Bomba, Lorenzo
AU - Capomaccio, Stefano
AU - Loor, Juan J.
AU - Ajmone-Marsan, Paolo
AU - Trevisi, Erminio
N1 - Publisher Copyright:
© 2019, Springer-Verlag GmbH Germany, part of Springer Nature.
PY - 2020/3/1
Y1 - 2020/3/1
N2 - Dairy cows during the transition period are faced with important physiological changes which include a dysfunctional immune system and an increased inflammatory state. New data are necessary to understand the key factors involved in the immune system regulation. Six dairy cows were sampled during transition period to investigate the leukocyte transcriptome changes and its relationship with blood biomarkers. Blood samples were collected at − 20 ± 2, − 3 ± 1, 3, and 7 days from parturition (DFP). Leukocyte transcriptome was analyzed by deep sequencing technology (Hiseq1000 Illumina, USA). Plasma was analyzed for metabolic biomarkers. Differentially expressed genes (DEG) were used to run an enrichment analysis through the Dynamic Impact Approach (DIA). Considering − 20 DFP as references time, the main KEGG impacted pathways were activated before calving (− 3 DFP) and were connected to lipid metabolism, lipid transport in plasma, and phagosome. The greatest differences were found after parturition with 281 DEG (179 upregulated and 102 downregulated). The activated pathways were mainly related to immunity and endocrine aspects, while metabolic pathways related to lipid and amino acid metabolism were inhibited. Plasma BHBA had a substantial inhibitory impact on KEGG pathways related to DNA replication and cell cycle, while plasma IL-1β had an inhibitory impact on fatty acid elongation in mitochondria and an activated impact in several pathways related to cellular energy metabolism. Overall, this study confirmed that many changes in lipid metabolism and immune competence of the circulating leukocytes occurred in dairy cow around calving. Interestingly, BHBA and IL-1β connected with the transcriptome.
AB - Dairy cows during the transition period are faced with important physiological changes which include a dysfunctional immune system and an increased inflammatory state. New data are necessary to understand the key factors involved in the immune system regulation. Six dairy cows were sampled during transition period to investigate the leukocyte transcriptome changes and its relationship with blood biomarkers. Blood samples were collected at − 20 ± 2, − 3 ± 1, 3, and 7 days from parturition (DFP). Leukocyte transcriptome was analyzed by deep sequencing technology (Hiseq1000 Illumina, USA). Plasma was analyzed for metabolic biomarkers. Differentially expressed genes (DEG) were used to run an enrichment analysis through the Dynamic Impact Approach (DIA). Considering − 20 DFP as references time, the main KEGG impacted pathways were activated before calving (− 3 DFP) and were connected to lipid metabolism, lipid transport in plasma, and phagosome. The greatest differences were found after parturition with 281 DEG (179 upregulated and 102 downregulated). The activated pathways were mainly related to immunity and endocrine aspects, while metabolic pathways related to lipid and amino acid metabolism were inhibited. Plasma BHBA had a substantial inhibitory impact on KEGG pathways related to DNA replication and cell cycle, while plasma IL-1β had an inhibitory impact on fatty acid elongation in mitochondria and an activated impact in several pathways related to cellular energy metabolism. Overall, this study confirmed that many changes in lipid metabolism and immune competence of the circulating leukocytes occurred in dairy cow around calving. Interestingly, BHBA and IL-1β connected with the transcriptome.
KW - Dairy cows
KW - Immune system
KW - Plasma metabolites
KW - RNA-Seq
KW - Transcriptomics
KW - Transition period
UR - http://www.scopus.com/inward/record.url?scp=85074602501&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85074602501&partnerID=8YFLogxK
U2 - 10.1007/s10142-019-00720-0
DO - 10.1007/s10142-019-00720-0
M3 - Article
C2 - 31654229
AN - SCOPUS:85074602501
SN - 1438-793X
VL - 20
SP - 293
EP - 305
JO - Functional and Integrative Genomics
JF - Functional and Integrative Genomics
IS - 2
ER -