TY - JOUR
T1 - Evaluation of an African swine fever (ASF) vaccine strategy incorporating priming with an alphavirus-expressed antigen followed by boosting with attenuated ASF virus
AU - Murgia, Maria V.
AU - Mogler, Mark
AU - Certoma, Andrea
AU - Green, Diane
AU - Monaghan, Paul
AU - Williams, David T.
AU - Rowland, Raymond R.R.
AU - Gaudreault, Natasha N.
N1 - Funding Information:
Funding This work was funded by the Kansas National Bio and Agro-Defense Facility (NBAF) Transition Fund. Partial support was from the Department of Homeland Security Center of Excellence for Emerging and Zoonotic Animal Diseases (CEEZAD) (Grant no. 2010-ST061-AG0001), and Kansas Biosciences Authority/CEEZAD matching funds (Award no. BG2428).
Publisher Copyright:
© 2018, Springer-Verlag GmbH Austria, part of Springer Nature.
PY - 2019/2/1
Y1 - 2019/2/1
N2 - In this study, an alphavirus vector platform was used to deliver replicon particles (RPs) expressing African swine fever virus (ASFV) antigens to swine. Alphavirus RPs expressing ASFV p30 (RP-30), p54 (RP-54) or pHA-72 (RP-sHA-p72) antigens were constructed and tested for expression in Vero cells and for immunogenicity in pigs. RP-30 showed the highest expression in Vero cells and was the most immunogenic in pigs, followed by RP-54 and RP-sHA-p72. Pigs primed with two doses of the RP-30 construct were then boosted with a naturally attenuated ASFV isolate, OURT88/3. Mapping of p30 identified an immunodominant region within the amino acid residues 111–130. However, the principal effect of the prime-boost was enhanced recognition of an epitope covered by the peptide sequence 61–110. The results suggest that a strategy incorporating priming with a vector-expressed antigen followed by boosting with an attenuated live virus may broaden the recognition of ASFV epitopes.
AB - In this study, an alphavirus vector platform was used to deliver replicon particles (RPs) expressing African swine fever virus (ASFV) antigens to swine. Alphavirus RPs expressing ASFV p30 (RP-30), p54 (RP-54) or pHA-72 (RP-sHA-p72) antigens were constructed and tested for expression in Vero cells and for immunogenicity in pigs. RP-30 showed the highest expression in Vero cells and was the most immunogenic in pigs, followed by RP-54 and RP-sHA-p72. Pigs primed with two doses of the RP-30 construct were then boosted with a naturally attenuated ASFV isolate, OURT88/3. Mapping of p30 identified an immunodominant region within the amino acid residues 111–130. However, the principal effect of the prime-boost was enhanced recognition of an epitope covered by the peptide sequence 61–110. The results suggest that a strategy incorporating priming with a vector-expressed antigen followed by boosting with an attenuated live virus may broaden the recognition of ASFV epitopes.
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U2 - 10.1007/s00705-018-4071-8
DO - 10.1007/s00705-018-4071-8
M3 - Article
C2 - 30367292
AN - SCOPUS:85055867125
SN - 0304-8608
VL - 164
SP - 359
EP - 370
JO - Archives of Virology
JF - Archives of Virology
IS - 2
ER -