@article{59b2771d5d02414e9cf512267d60183c,
title = "Evaluating osteogenic effects associated with the incorporation of ascorbic acid in mineralized collagen scaffolds",
abstract = "Current treatments for craniomaxillofacial (CMF) defects motivate the design of instructive biomaterials that can promote osteogenic healing of complex bone defects. We report methods to promote in vitro osteogenesis of human mesenchymal stem cells (hMSCs) within a model mineralized collagen scaffold via the incorporation of ascorbic acid (vitamin C), a key factor in collagen biosynthesis and bone mineralization. An addition of 5 w/v% ascorbic acid into the base mineralized collagen scaffold significantly changes key morphology characteristics including porosity, macrostructure, and microstructure. This modification promotes hMSC metabolic activity, ALP activity, and hMSC-mediated deposition of calcium and phosphorous. Additionally, the incorporation of ascorbic acid influences osteogenic gene expression (BMP-2, RUNX2, COL1A2) and delays the expression of genes associated with osteoclast activity and bone resorption (OPN, CTSK), though it reduces the secretion of OPG. Together, these findings highlight ascorbic acid as a relevant component for mineralized collagen scaffold design to promote osteogenic differentiation and new bone formation for improved CMF outcomes.",
keywords = "ascorbic acid, bone, collagen scaffolds, craniomaxillofacial, mesenchymal stem cells, osteogenesis",
author = "Dewey, {Marley J.} and Timmer, {Kyle B.} and Ashley Blystone and Crislyn Lu and Harley, {Brendan A.C.}",
note = "We would like to acknowledge the following institutes for access to their facilities and services: the School of Chemical Sciences Microanalysis Laboratory, the Carl R. Woese Institute for Genomic Biology, the Roy J. Carver Biotechnology Center, and the Microscopy Suite of the Imaging Technology Group at the Beckman Institute for Advanced Science and Technology, located at the University of Illinois. The authors also acknowledge assistance from the School of Chemical Science Microanalysis Lab for assistance with inductively coupled plasma mass spectrometry/optical emission spectrometry. Research reported in this publication was supported by the National Institute of Dental and Craniofacial Research of the National Institutes of Health under Award Number R01 DE030491 (BACH) as well as by the National Institute of Arthritis and Musculoskeletal and Skin Diseases under Award Number R01 AR077858 (BACH). We are also grateful for funds provided by the NSF Graduate Research Fellowship DGE‐1144245 (MJD) to perform this research. The interpretations and conclusions presented are those of the authors and are not necessarily endorsed by the National Institutes of Health or the National Science Foundation. We would like to acknowledge the following institutes for access to their facilities and services: the School of Chemical Sciences Microanalysis Laboratory, the Carl R. Woese Institute for Genomic Biology, the Roy J. Carver Biotechnology Center, and the Microscopy Suite of the Imaging Technology Group at the Beckman Institute for Advanced Science and Technology, located at the University of Illinois. The authors also acknowledge assistance from the School of Chemical Science Microanalysis Lab for assistance with inductively coupled plasma mass spectrometry/optical emission spectrometry. Research reported in this publication was supported by the National Institute of Dental and Craniofacial Research of the National Institutes of Health under Award Number R01 DE030491 (BACH) as well as by the National Institute of Arthritis and Musculoskeletal and Skin Diseases under Award Number R01 AR077858 (BACH). We are also grateful for funds provided by the NSF Graduate Research Fellowship DGE-1144245 (MJD) to perform this research. The interpretations and conclusions presented are those of the authors and are not necessarily endorsed by the National Institutes of Health or the National Science Foundation.",
year = "2024",
month = mar,
doi = "10.1002/jbm.a.37628",
language = "English (US)",
volume = "112",
pages = "336--347",
journal = "Journal of Biomedical Materials Research - Part A",
issn = "1549-3296",
publisher = "John Wiley & Sons, Ltd.",
number = "3",
}