Evaluating osteogenic effects associated with the incorporation of ascorbic acid in mineralized collagen scaffolds

Marley J. Dewey, Kyle B. Timmer, Ashley Blystone, Crislyn Lu, Brendan A.C. Harley

Research output: Contribution to journalArticlepeer-review

Abstract

Current treatments for craniomaxillofacial (CMF) defects motivate the design of instructive biomaterials that can promote osteogenic healing of complex bone defects. We report methods to promote in vitro osteogenesis of human mesenchymal stem cells (hMSCs) within a model mineralized collagen scaffold via the incorporation of ascorbic acid (vitamin C), a key factor in collagen biosynthesis and bone mineralization. An addition of 5 w/v% ascorbic acid into the base mineralized collagen scaffold significantly changes key morphology characteristics including porosity, macrostructure, and microstructure. This modification promotes hMSC metabolic activity, ALP activity, and hMSC-mediated deposition of calcium and phosphorous. Additionally, the incorporation of ascorbic acid influences osteogenic gene expression (BMP-2, RUNX2, COL1A2) and delays the expression of genes associated with osteoclast activity and bone resorption (OPN, CTSK), though it reduces the secretion of OPG. Together, these findings highlight ascorbic acid as a relevant component for mineralized collagen scaffold design to promote osteogenic differentiation and new bone formation for improved CMF outcomes.

Original languageEnglish (US)
Pages (from-to)336-347
Number of pages12
JournalJournal of Biomedical Materials Research - Part A
Volume112
Issue number3
DOIs
StatePublished - Mar 2024

Keywords

  • ascorbic acid
  • bone
  • collagen scaffolds
  • craniomaxillofacial
  • mesenchymal stem cells
  • osteogenesis

ASJC Scopus subject areas

  • Ceramics and Composites
  • Metals and Alloys
  • Biomedical Engineering
  • Biomaterials

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