Evaluating genetic relationships in seed impatiens, Impatiens walleriana, using AFLP profiling

J. Carr, S. S. Korban

Research output: Contribution to journalArticle

Abstract

Although seed impatiens is the leading bedding plant in the US, little work has been conducted on evaluating the genetic variability present in this crop. In this study, amplified fragment length polymorphism technology was utilized to determine the level of polymorphism present across 20 commercial cultivars of seed impatiens, to assess their genetic variability, and to investigate their genetic relatedness. A total of eight EcoRI and MseI primer combinations were used for polymerase chain reaction amplification. Fluorescence-labelled amplification products were subjected to electrophoresis and then analysed using an automated sequencer. High levels of polymorphism were detected among all 20 cultivars for all primer combinations tested. Gower's Genetic Dissimilarity estimates for the entire set of cultivars ranged from 1.000 to 0.316. The dendogram generated from these dissimilarity data revealed a number of groupings, including one major division which placed all of the cultivars into one of two groups. The implications of these results on genetic variability, genotypic relationships and genetic diversity in seed impatiens is discussed.

Original languageEnglish (US)
Pages (from-to)577-581
Number of pages5
JournalPlant Breeding
Volume123
Issue number6
DOIs
StatePublished - Dec 1 2004

Keywords

  • Amplified fragment length polymorphism
  • Genetic relatedness
  • Impatiens walleriana
  • Molecular markers
  • Polymorphism

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Genetics
  • Plant Science

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