Estrogen receptor regulation of carbonic anhydrase XII through a distal enhancer in breast cancer

Daniel H. Barnett, Shubin Sheng, Tze Howe Charn, Abdul Waheed, William S. Sly, Chin Yo Lin, Edison T. Liu, Benita S. Katzenellenbogen

Research output: Contribution to journalArticle

Abstract

The expression of carbonic anhydrase XII (CA12), a gene that encodes a zinc metalloenzyme responsible for acidification of the microenvironment of cancer cells, is highly correlated with estrogen receptor α (ERα) in human breast tumors. Here, we show that CA12 is robustly regulated by estrogen via ERA in breast cancer cells, and that this regulation involves a distal estrogen-responsive enhancer region. Upon the addition of estradiol, ERα binds directly to this distal enhancer in vivo, resulting in the recruitment of RNA polymerase II and steroid receptor coactivators SRC-2 and SRC-3, and changes in histone acetylation. Mutagenesis of an imperfect estrogen-responsive element within this enhancer region abolishes estrogen-dependent activity, and chromosome conformation capture and chromatin immunoprecipitation assays show that this distal enhancer communicates with the transcriptional start site of the CA12 gene via intrachromosomal looping upon hormone treatment. This distal enhancer element is observed in the homologous mouse genomic sequence, and the expression of the mouse homologue, Car12, is rapidly and robustly stimulated by estradiol in the mouse uterus in vivo, suggesting that the ER regulation of CA12 is mechanistically and evolutionarily conserved. Our findings highlight the crucial role of ER in the regulation of the CA12 gene, and provide insight into the transcriptional regulatory mechanism that accounts for the strong association of CA12 and ER in human breast cancers.

Original languageEnglish (US)
Pages (from-to)3505-3515
Number of pages11
JournalCancer Research
Volume68
Issue number9
DOIs
StatePublished - May 1 2008

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Estrogen Receptors
Breast Neoplasms
Estrogens
Nuclear Receptor Coactivator 2
Genes
Estradiol Receptors
Cellular Microenvironment
Tumor Microenvironment
RNA Polymerase II
Chromatin Immunoprecipitation
Acetylation
Mutagenesis
Histones
Uterus
Zinc
Estradiol
Chromosomes
carbonic anhydrase XII
Hormones

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Estrogen receptor regulation of carbonic anhydrase XII through a distal enhancer in breast cancer. / Barnett, Daniel H.; Sheng, Shubin; Charn, Tze Howe; Waheed, Abdul; Sly, William S.; Lin, Chin Yo; Liu, Edison T.; Katzenellenbogen, Benita S.

In: Cancer Research, Vol. 68, No. 9, 01.05.2008, p. 3505-3515.

Research output: Contribution to journalArticle

Barnett, Daniel H. ; Sheng, Shubin ; Charn, Tze Howe ; Waheed, Abdul ; Sly, William S. ; Lin, Chin Yo ; Liu, Edison T. ; Katzenellenbogen, Benita S. / Estrogen receptor regulation of carbonic anhydrase XII through a distal enhancer in breast cancer. In: Cancer Research. 2008 ; Vol. 68, No. 9. pp. 3505-3515.
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