Estrogen receptor activation function 1 works by binding p160 coactivator proteins

P. Webb; P. Nguyen; J. Shinsako; C. Anderson; W. Feng; M. P. Nguyen; D. Chen; S. M. Huang; S. Subramanian; E. McKinerney; B. S. Katzenellenbogen; M. R. Stallcup; P. J. Kushner

doi:10.1210/mend.12.10.0185

Estrogen receptor activation function 1 works by binding p160 coactivator proteins

P. Webb, P. Nguyen, J. Shinsako, C. Anderson, W. Feng, M. P. Nguyen, D. Chen, S. M. Huang, S. Subramanian, E. McKinerney, B. S. Katzenellenbogen, M. R. Stallcup, P. J. Kushner

Molecular and Integrative Physiology

Research output: Contribution to journal › Article

Abstract

Estrogen receptor-α contains two transactivation functions, a weak constitutive activation function (AF-1) and a hormone-dependent activation function (AF-2). AF-2 works by recruiting a large coactivator complex, composed of one or more p160s, CREB-binding protein (CBP)/p300, and P/CAF (p300 and CBP-associated factor), via direct contacts with the p160s. We report here that independent AF-1 activity also requires p160 contacts. Unlike AF-2, which binds signature NR boxes in the center of the p160 molecule, AF-1 binds to sequences near the p160 C terminus. We propose that the ability of AF-1 and AF-2 to interact with separate surfaces of the same coactivator is important for the ability of these transactivation functions to synergize.

Original language	English (US)
Pages (from-to)	1605-1618
Number of pages	14
Journal	Molecular Endocrinology
Volume	12
Issue number	10
DOIs	https://doi.org/10.1210/mend.12.10.0185
State	Published - 1998

ASJC Scopus subject areas

Molecular Biology
Endocrinology

Access to Document

10.1210/mend.12.10.0185

Fingerprint Dive into the research topics of 'Estrogen receptor activation function 1 works by binding p160 coactivator proteins'. Together they form a unique fingerprint.

Cite this

Webb, P., Nguyen, P., Shinsako, J., Anderson, C., Feng, W., Nguyen, M. P., Chen, D., Huang, S. M., Subramanian, S., McKinerney, E., Katzenellenbogen, B. S., Stallcup, M. R., & Kushner, P. J. (1998). Estrogen receptor activation function 1 works by binding p160 coactivator proteins. Molecular Endocrinology, 12(10), 1605-1618. https://doi.org/10.1210/mend.12.10.0185

Estrogen receptor activation function 1 works by binding p160 coactivator proteins. / Webb, P.; Nguyen, P.; Shinsako, J.; Anderson, C.; Feng, W.; Nguyen, M. P.; Chen, D.; Huang, S. M.; Subramanian, S.; McKinerney, E.; Katzenellenbogen, B. S.; Stallcup, M. R.; Kushner, P. J.

In: Molecular Endocrinology, Vol. 12, No. 10, 1998, p. 1605-1618.

Research output: Contribution to journal › Article

@article{b115300291d246faa40a5b4b5130e8ae,

title = "Estrogen receptor activation function 1 works by binding p160 coactivator proteins",

abstract = "Estrogen receptor-α contains two transactivation functions, a weak constitutive activation function (AF-1) and a hormone-dependent activation function (AF-2). AF-2 works by recruiting a large coactivator complex, composed of one or more p160s, CREB-binding protein (CBP)/p300, and P/CAF (p300 and CBP-associated factor), via direct contacts with the p160s. We report here that independent AF-1 activity also requires p160 contacts. Unlike AF-2, which binds signature NR boxes in the center of the p160 molecule, AF-1 binds to sequences near the p160 C terminus. We propose that the ability of AF-1 and AF-2 to interact with separate surfaces of the same coactivator is important for the ability of these transactivation functions to synergize.",

author = "P. Webb and P. Nguyen and J. Shinsako and C. Anderson and W. Feng and Nguyen, {M. P.} and D. Chen and Huang, {S. M.} and S. Subramanian and E. McKinerney and Katzenellenbogen, {B. S.} and Stallcup, {M. R.} and Kushner, {P. J.}",

year = "1998",

doi = "10.1210/mend.12.10.0185",

language = "English (US)",

volume = "12",

pages = "1605--1618",

journal = "Molecular Endocrinology",

issn = "0888-8809",

publisher = "The Endocrine Society",

number = "10",

}

TY - JOUR

T1 - Estrogen receptor activation function 1 works by binding p160 coactivator proteins

AU - Webb, P.

AU - Nguyen, P.

AU - Shinsako, J.

AU - Anderson, C.

AU - Feng, W.

AU - Nguyen, M. P.

AU - Chen, D.

AU - Huang, S. M.

AU - Subramanian, S.

AU - McKinerney, E.

AU - Katzenellenbogen, B. S.

AU - Stallcup, M. R.

AU - Kushner, P. J.

PY - 1998

Y1 - 1998

N2 - Estrogen receptor-α contains two transactivation functions, a weak constitutive activation function (AF-1) and a hormone-dependent activation function (AF-2). AF-2 works by recruiting a large coactivator complex, composed of one or more p160s, CREB-binding protein (CBP)/p300, and P/CAF (p300 and CBP-associated factor), via direct contacts with the p160s. We report here that independent AF-1 activity also requires p160 contacts. Unlike AF-2, which binds signature NR boxes in the center of the p160 molecule, AF-1 binds to sequences near the p160 C terminus. We propose that the ability of AF-1 and AF-2 to interact with separate surfaces of the same coactivator is important for the ability of these transactivation functions to synergize.

AB - Estrogen receptor-α contains two transactivation functions, a weak constitutive activation function (AF-1) and a hormone-dependent activation function (AF-2). AF-2 works by recruiting a large coactivator complex, composed of one or more p160s, CREB-binding protein (CBP)/p300, and P/CAF (p300 and CBP-associated factor), via direct contacts with the p160s. We report here that independent AF-1 activity also requires p160 contacts. Unlike AF-2, which binds signature NR boxes in the center of the p160 molecule, AF-1 binds to sequences near the p160 C terminus. We propose that the ability of AF-1 and AF-2 to interact with separate surfaces of the same coactivator is important for the ability of these transactivation functions to synergize.

UR - http://www.scopus.com/inward/record.url?scp=0032230350&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032230350&partnerID=8YFLogxK

U2 - 10.1210/mend.12.10.0185

DO - 10.1210/mend.12.10.0185

M3 - Article

C2 - 9773983

AN - SCOPUS:0032230350

VL - 12

SP - 1605

EP - 1618

JO - Molecular Endocrinology

JF - Molecular Endocrinology

SN - 0888-8809

IS - 10

ER -