Estimation of total phenolic content and other oxidation substrates in plant tissues using Folin-Ciocalteu reagent

Elizabeth A. Ainsworth, Kelly M. Gillespie

Research output: Contribution to journalArticlepeer-review

Abstract

Non-structural phenolic compounds perform a variety of functions in plants, including acting as antioxidants. We describe a microplate-adapted colorimetric total phenolics assay that utilizes Folin-Ciocalteu (F-C) reagent. The F-C assay relies on the transfer of electrons in alkaline medium from phenolic compounds to phosphomolybdic/ phosphotungstic acid complexes, which are determined spectroscopically at 765 nm. Although the electron transfer reaction is not specific for phenolic compounds, the extraction procedure eliminates approximately 85% of ascorbic acid and other potentially interfering compounds. This assay is performed in microcentrifuge tubes and assessed in a 96-well plate reader. At least 64 samples can be processed in 1 d.

Original languageEnglish (US)
Pages (from-to)875-877
Number of pages3
JournalNature Protocols
Volume2
Issue number4
DOIs
StatePublished - Apr 1 2007

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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