Establishment of Restraint Stress–induced Anorexia and Social Isolation–induced Anorexia Mouse Models

Ingrid Camila Possa-Paranhos, Kerem Catalbas, Jared Butts, Kyle O’Berry, Patrick Sweeney

Research output: Contribution to journalArticlepeer-review


Anorexia nervosa (AN) is a devastating neuropsychiatric disease with a prevalence rate of approximately 0.3%–1% among women and morbidity and mortality rates among the highest of all neuropsychiatric disorders. The disease etiology is complex but primarily characterized by reduced food intake and body weight, and intense anxiety and fear associated with gaining weight. Existing rodent models of AN are useful and capture features of the disease, but either require specialized genetic mouse models or are difficult to implement in mice. Here, we describe two simple mouse models of stress-induced anorexia that are easy to implement in basic research labs, and capture core features associated with AN, such as reduced food intake in the context of social/physical stress and increased anxiety-related behavior. These protocols provide reproducible and robust assays for stress-induced anorexia and may be implemented with additional assays to probe the neural circuitry mediating the effects of psychological stress on feeding in mice.

Original languageEnglish (US)
Article numbere4597
Issue number2
StatePublished - Jan 20 2023


  • Anorexia nervosa
  • Eating disorders
  • Feeding
  • Mouse models
  • Neural circuits
  • Restraint Stress–induced anorexia

ASJC Scopus subject areas

  • General Immunology and Microbiology
  • General Biochemistry, Genetics and Molecular Biology
  • General Neuroscience
  • Plant Science


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