Erythrocyte structure and dynamics quantified by Hilbert phase microscopy

Gabriel Popescu, Takahiro Ikeda, Catherine A. Best, Kamran Badizadegan, Ramachandra R. Dasari, Michael S. Feld

Research output: Contribution to journalArticlepeer-review

Abstract

We present a new quantitative method for investigating red blood cell morphology and dynamics. The instrument integrates quantitative phase microscopy with an inverted microscope, which makes it particularly suitable for the noninvasive assessment of live erythrocytes. In particular, we demonstrate the ability of this approach to quantify noninvasively cell volume and dynamic morphology. The subnanometer path-length sensitivity at the millisecond time scales is exemplified by measuring the hemoglobin flow out of the cell during hemolysis.

Original languageEnglish (US)
Article number060503
JournalJournal of biomedical optics
Volume10
Issue number6
DOIs
StatePublished - Nov 2005
Externally publishedYes

Keywords

  • Cells
  • Medical imaging
  • Microscopy
  • Phase retrieval

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials
  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging
  • Radiological and Ultrasound Technology
  • Clinical Biochemistry

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