Equipment Setup for Fluorescence Imaging with One-Nanometer Accuracy (FIONA)

Paul R Selvin; Tyler Lougheed; Melinda Tonks Hoffman; Hyokeun Park; Hamza Balci; Benjamin H Blehm; Erdal Toprak

doi:10.1101/pdb.ip45

Equipment Setup for Fluorescence Imaging with One-Nanometer Accuracy (FIONA)

Paul R Selvin, Tyler Lougheed, Melinda Tonks Hoffman, Hyokeun Park, Hamza Balci, Benjamin H Blehm, Erdal Toprak

Research output: Contribution to journal › Article › peer-review

Abstract

INTRODUCTION: FIONA, short for fluorescence imaging with one-nanometer accuracy, is a simple method for achieving localization of single (or single groups of) fluorophores with nanometer accuracy in the xy plane. This article describes procedures for setting up the equipment necessary for FIONA and achieving total internal reflection (TIR).

Original language	English (US)
Pages (from-to)	pdb.ip45
Journal	Cold Spring Harbor Protocols
Volume	2007
DOIs	https://doi.org/10.1101/pdb.ip45
State	Published - Oct 1 2007

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title = "Equipment Setup for Fluorescence Imaging with One-Nanometer Accuracy (FIONA)",

abstract = "INTRODUCTION: FIONA, short for fluorescence imaging with one-nanometer accuracy, is a simple method for achieving localization of single (or single groups of) fluorophores with nanometer accuracy in the xy plane. This article describes procedures for setting up the equipment necessary for FIONA and achieving total internal reflection (TIR).",

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AU - Selvin, Paul R

AU - Lougheed, Tyler

AU - Tonks Hoffman, Melinda

AU - Park, Hyokeun

AU - Balci, Hamza

AU - Blehm, Benjamin H

AU - Toprak, Erdal

PY - 2007/10/1

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N2 - INTRODUCTION: FIONA, short for fluorescence imaging with one-nanometer accuracy, is a simple method for achieving localization of single (or single groups of) fluorophores with nanometer accuracy in the xy plane. This article describes procedures for setting up the equipment necessary for FIONA and achieving total internal reflection (TIR).

AB - INTRODUCTION: FIONA, short for fluorescence imaging with one-nanometer accuracy, is a simple method for achieving localization of single (or single groups of) fluorophores with nanometer accuracy in the xy plane. This article describes procedures for setting up the equipment necessary for FIONA and achieving total internal reflection (TIR).

U2 - 10.1101/pdb.ip45

DO - 10.1101/pdb.ip45

M3 - Article

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SN - 1559-6095

VL - 2007

SP - pdb.ip45

JO - Cold Spring Harbor Protocols

JF - Cold Spring Harbor Protocols

ER -

Equipment Setup for Fluorescence Imaging with One-Nanometer Accuracy (FIONA)

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