Epitope mapping of African swine fever virus (ASFV) structural protein, p54

Vlad Petrovan, Maria V. Murgia, Ping Wu, Andre D. Lowe, Wei Jia, Raymond R.R. Rowland

Research output: Contribution to journalArticle

Abstract

In the absence of a vaccine for African swine fever virus (ASFV), diagnostic tools are critical for early detection and implementation of control measures. Along with other immunogenic proteins, p54 is a good serological target for conducting ASF detection and surveillance. In this study, a panel of 12 mouse monoclonal antibodies (mAbs) was prepared against a baculovirus-expressed p54(60–178) polypeptide. Further screening showed that five mAbs were positive for reactivity against ASFV-infected cells and recombinant p54 proteins. Mapping studies using five polypeptides and 12 oligopeptides, showed that mAb #154-1 recognized a conserved polypeptide sequence, p54(65–75), and was placed into Group 1. Mabs #143-1 and #7 recognized a region covered by p54(93–113) and were placed into Group 2. Group 3 consisted of mAbs #101 and #117, which recognized p54(118–127). Sera from pigs infected with the low virulent OURT 88/3 strain recognized the same p54 region covered by the Group 3 mAbs. When tested in a neutralization format, only mAb #143-1 showed neutralization activity above background. Together, the results identify important antigenic and immunogenic regions located on p54, which provide new tools for improving ASFV diagnostics.

Original languageEnglish (US)
Article number197871
JournalVirus Research
Volume279
DOIs
StatePublished - Apr 2 2020
Externally publishedYes

Keywords

  • African swine fever virus
  • Epitope mapping
  • Monoclonal antibodies
  • p54

ASJC Scopus subject areas

  • Cancer Research
  • Virology
  • Infectious Diseases

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