Epigenetic Process Monitoring in Live Cultures with Peptide Biosensors

Nur P. Damayanti, Kevin Buno, Sherry L. Voytik Harbin, Joseph Maria Kumar Irudayaraj

Research output: Contribution to journalArticle

Abstract

Acetyltransferase is a member of the transferase group responsible for transferring an acetyl group from acetyl-CoA to amino group of a histone lysine residue. Past efforts on histone acetylation monitoring involved biochemical analysis that do not provide spatiotemporal information in a dynamic format. We propose a novel approach to monitor acetyltransferase acetylation in live single cells using time correlated single photon counting fluorescence lifetime imaging (TCSPC-FLIM) with peptide biosensors. Utilizing 2D and 3D cultures we show that the peptide sensor has a specific response to acetyltransferase enzyme activity in a fluorescence lifetime dependent manner (P < 0.001). Our FLIM biosensor concept enables real-time longitudinal measurement of acetylation activity with high spatial and temporal resolution in live single cells to monitor cell function or evaluate drug effects to treat cancer or neurological diseases.

Original languageEnglish (US)
Pages (from-to)562-565
Number of pages4
JournalACS Sensors
Volume4
Issue number3
DOIs
StatePublished - Mar 22 2019

Fingerprint

acetylation
Acetylation
Acetyltransferases
Process monitoring
bioinstrumentation
Biosensors
Peptides
peptides
Histones
cells
Fluorescence
life (durability)
fluorescence
enzyme activity
Acetyl Coenzyme A
lysine
Enzyme activity
Transferases
temporal resolution
Lysine

Keywords

  • FLIM
  • acetylation
  • live cultures
  • peptide sensor
  • real-time detection

ASJC Scopus subject areas

  • Bioengineering
  • Instrumentation
  • Process Chemistry and Technology
  • Fluid Flow and Transfer Processes

Cite this

Epigenetic Process Monitoring in Live Cultures with Peptide Biosensors. / Damayanti, Nur P.; Buno, Kevin; Voytik Harbin, Sherry L.; Irudayaraj, Joseph Maria Kumar.

In: ACS Sensors, Vol. 4, No. 3, 22.03.2019, p. 562-565.

Research output: Contribution to journalArticle

Damayanti, Nur P. ; Buno, Kevin ; Voytik Harbin, Sherry L. ; Irudayaraj, Joseph Maria Kumar. / Epigenetic Process Monitoring in Live Cultures with Peptide Biosensors. In: ACS Sensors. 2019 ; Vol. 4, No. 3. pp. 562-565.
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