Epigenetic Process Monitoring in Live Cultures with Peptide Biosensors

Nur P. Damayanti; Kevin Buno; Sherry L. Voytik Harbin; Joseph M.K. Irudayaraj

doi:10.1021/acssensors.8b01134

Epigenetic Process Monitoring in Live Cultures with Peptide Biosensors

Nur P. Damayanti, Kevin Buno, Sherry L. Voytik Harbin, Joseph M.K. Irudayaraj

Research output: Contribution to journal › Article › peer-review

Abstract

Acetyltransferase is a member of the transferase group responsible for transferring an acetyl group from acetyl-CoA to amino group of a histone lysine residue. Past efforts on histone acetylation monitoring involved biochemical analysis that do not provide spatiotemporal information in a dynamic format. We propose a novel approach to monitor acetyltransferase acetylation in live single cells using time correlated single photon counting fluorescence lifetime imaging (TCSPC-FLIM) with peptide biosensors. Utilizing 2D and 3D cultures we show that the peptide sensor has a specific response to acetyltransferase enzyme activity in a fluorescence lifetime dependent manner (P < 0.001). Our FLIM biosensor concept enables real-time longitudinal measurement of acetylation activity with high spatial and temporal resolution in live single cells to monitor cell function or evaluate drug effects to treat cancer or neurological diseases.

Original language	English (US)
Pages (from-to)	562-565
Number of pages	4
Journal	ACS Sensors
Volume	4
Issue number	3
DOIs	https://doi.org/10.1021/acssensors.8b01134
State	Published - Mar 22 2019

Keywords

FLIM
acetylation
live cultures
peptide sensor
real-time detection

ASJC Scopus subject areas

Bioengineering
Instrumentation
Process Chemistry and Technology
Fluid Flow and Transfer Processes

Online availability

10.1021/acssensors.8b01134

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title = "Epigenetic Process Monitoring in Live Cultures with Peptide Biosensors",

abstract = "Acetyltransferase is a member of the transferase group responsible for transferring an acetyl group from acetyl-CoA to amino group of a histone lysine residue. Past efforts on histone acetylation monitoring involved biochemical analysis that do not provide spatiotemporal information in a dynamic format. We propose a novel approach to monitor acetyltransferase acetylation in live single cells using time correlated single photon counting fluorescence lifetime imaging (TCSPC-FLIM) with peptide biosensors. Utilizing 2D and 3D cultures we show that the peptide sensor has a specific response to acetyltransferase enzyme activity in a fluorescence lifetime dependent manner (P < 0.001). Our FLIM biosensor concept enables real-time longitudinal measurement of acetylation activity with high spatial and temporal resolution in live single cells to monitor cell function or evaluate drug effects to treat cancer or neurological diseases.",

keywords = "FLIM, acetylation, live cultures, peptide sensor, real-time detection",

author = "Damayanti, {Nur P.} and Kevin Buno and {Voytik Harbin}, {Sherry L.} and Irudayaraj, {Joseph M.K.}",

note = "The authors acknowledge the grant from W. M. Keck Foundation to J.I. J.I. gratefully acknowledges funding in part from the Purdue University Center for Cancer Research, NIH Grant P30CA023168 and the Indiana CTSI Grant 358 UL1TR001108 from NIH.",

year = "2019",

month = mar,

day = "22",

doi = "10.1021/acssensors.8b01134",

language = "English (US)",

volume = "4",

pages = "562--565",

journal = "ACS Sensors",

issn = "2379-3694",

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AU - Damayanti, Nur P.

AU - Buno, Kevin

AU - Voytik Harbin, Sherry L.

AU - Irudayaraj, Joseph M.K.

N1 - The authors acknowledge the grant from W. M. Keck Foundation to J.I. J.I. gratefully acknowledges funding in part from the Purdue University Center for Cancer Research, NIH Grant P30CA023168 and the Indiana CTSI Grant 358 UL1TR001108 from NIH.

PY - 2019/3/22

Y1 - 2019/3/22

N2 - Acetyltransferase is a member of the transferase group responsible for transferring an acetyl group from acetyl-CoA to amino group of a histone lysine residue. Past efforts on histone acetylation monitoring involved biochemical analysis that do not provide spatiotemporal information in a dynamic format. We propose a novel approach to monitor acetyltransferase acetylation in live single cells using time correlated single photon counting fluorescence lifetime imaging (TCSPC-FLIM) with peptide biosensors. Utilizing 2D and 3D cultures we show that the peptide sensor has a specific response to acetyltransferase enzyme activity in a fluorescence lifetime dependent manner (P < 0.001). Our FLIM biosensor concept enables real-time longitudinal measurement of acetylation activity with high spatial and temporal resolution in live single cells to monitor cell function or evaluate drug effects to treat cancer or neurological diseases.

AB - Acetyltransferase is a member of the transferase group responsible for transferring an acetyl group from acetyl-CoA to amino group of a histone lysine residue. Past efforts on histone acetylation monitoring involved biochemical analysis that do not provide spatiotemporal information in a dynamic format. We propose a novel approach to monitor acetyltransferase acetylation in live single cells using time correlated single photon counting fluorescence lifetime imaging (TCSPC-FLIM) with peptide biosensors. Utilizing 2D and 3D cultures we show that the peptide sensor has a specific response to acetyltransferase enzyme activity in a fluorescence lifetime dependent manner (P < 0.001). Our FLIM biosensor concept enables real-time longitudinal measurement of acetylation activity with high spatial and temporal resolution in live single cells to monitor cell function or evaluate drug effects to treat cancer or neurological diseases.

KW - FLIM

KW - acetylation

KW - live cultures

KW - peptide sensor

KW - real-time detection

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JO - ACS Sensors

JF - ACS Sensors

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ER -

Epigenetic Process Monitoring in Live Cultures with Peptide Biosensors

Abstract

Keywords

ASJC Scopus subject areas

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