TY - JOUR
T1 - Epigenetic alterations associated with dexamethasone sodium phosphate through DNMT and TET in RPE cells
AU - Liu, Wenjie
AU - Mohan, Sruthi Priya
AU - Nagaraj, Nareshkumar Ragavachetty
AU - Jaganathan, Shyam Sundar
AU - Wen, Yi
AU - Ramasubramanyan, Sharada
AU - Irudayaraj, Joseph
N1 - Publisher Copyright:
© 2021 Molecular Vision.
PY - 2021
Y1 - 2021
N2 - Purpose: To elucidate the mechanism behind epigenetic alteration associated with dexamethasone (DEX) sodium phosphate treatment. Methods: We performed enzyme-linked immunosorbent assay to quantify changes in global DNA methylation and hydroxymethylation, quantitative real-time PCR (qRT-PCR) of the DNA methylation- and hydroxymethylation-related gene, in vitro DNA methyltransferase (DNMT) enzymatic activity assays with purified DNMTs, and DNA hydroxymethylation pattern with super-resolution imaging. Results: We identified global DNA hypomethylation and hyper-hydroxymethylation upon DEX treatment, associated with aberrant mRNA expression levels of DNMT and ten-eleven translocation (TET) proteins. Additionally, DEX exposure could directly hinder DNMT activities. Conclusions: We showed that DEX-induced epigenetic alterations are linked to aberrant DNMT and TET expression, potentially through an essential role of DNMT.
AB - Purpose: To elucidate the mechanism behind epigenetic alteration associated with dexamethasone (DEX) sodium phosphate treatment. Methods: We performed enzyme-linked immunosorbent assay to quantify changes in global DNA methylation and hydroxymethylation, quantitative real-time PCR (qRT-PCR) of the DNA methylation- and hydroxymethylation-related gene, in vitro DNA methyltransferase (DNMT) enzymatic activity assays with purified DNMTs, and DNA hydroxymethylation pattern with super-resolution imaging. Results: We identified global DNA hypomethylation and hyper-hydroxymethylation upon DEX treatment, associated with aberrant mRNA expression levels of DNMT and ten-eleven translocation (TET) proteins. Additionally, DEX exposure could directly hinder DNMT activities. Conclusions: We showed that DEX-induced epigenetic alterations are linked to aberrant DNMT and TET expression, potentially through an essential role of DNMT.
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M3 - Article
C2 - 34924744
AN - SCOPUS:85122181661
SN - 1090-0535
VL - 27
SP - 643
EP - 655
JO - Molecular Vision
JF - Molecular Vision
ER -