Abstract

Multiple scattering and absorption limit the depth at which biological tissues can be imaged with light. In thick unlabeled specimens, multiple scattering randomizes the phase of the field and absorption attenuates light that travels long optical paths. These obstacles limit the performance of transmission imaging. To mitigate these challenges, we developed an epi-illumination gradient light interference microscope (epi-GLIM) as a label-free phase imaging modality applicable to bulk or opaque samples. Epi-GLIM enables studying turbid structures that are hundreds of microns thick and otherwise opaque to transmitted light. We demonstrate this approach with a variety of man-made and biological samples that are incompatible with imaging in a transmission geometry: semiconductors wafers, specimens on opaque and birefringent substrates, cells in microplates, and bulk tissues. We demonstrate that the epi-GLIM data can be used to solve the inverse scattering problem and reconstruct the tomography of single cells and model organisms.

Original languageEnglish (US)
Article number4691
JournalNature communications
Volume10
Issue number1
DOIs
StatePublished - Dec 1 2019

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Interference Microscopy
Light interference
Lighting
Microscopic examination
Multiple scattering
illumination
microscopy
interference
Imaging techniques
Light
gradients
Microscopes
Tissue
microscopes
Light absorption
Tomography
Labels
inverse scattering
electromagnetic absorption
optical paths

ASJC Scopus subject areas

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

Cite this

Epi-illumination gradient light interference microscopy for imaging opaque structures. / Kandel, Mikhail E.; Hu, Chenfei; Naseri Kouzehgarani, Ghazal; Min, Eunjung; Sullivan, Kathryn Michele; Kong, Hyunjoon; Li, Jennifer M.; Robson, Drew N.; Gillette, Martha U.; Best-Popescu, Catherine; Popescu, Gabriel.

In: Nature communications, Vol. 10, No. 1, 4691, 01.12.2019.

Research output: Contribution to journalArticle

Kandel, Mikhail E. ; Hu, Chenfei ; Naseri Kouzehgarani, Ghazal ; Min, Eunjung ; Sullivan, Kathryn Michele ; Kong, Hyunjoon ; Li, Jennifer M. ; Robson, Drew N. ; Gillette, Martha U. ; Best-Popescu, Catherine ; Popescu, Gabriel. / Epi-illumination gradient light interference microscopy for imaging opaque structures. In: Nature communications. 2019 ; Vol. 10, No. 1.
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