Epi-illumination diffraction phase microscopy with white light

C. Edwards, B. Bhaduri, B. G. Griffin, L. L. Goddard, G. Popescu

Research output: Contribution to journalArticle

Abstract

We demonstrate the first reflection-based epi-illumination diffraction phase microscope with white light (epi-wDPM). The epi-wDPM system combines the off-axis, common-path, and white light approaches, in a reflection geometry enabling sub-nanometer spatial and temporal noise levels, while providing single-shot acquisition for opaque samples. We verified the epi-wDPM results by measuring control samples with known dimensions and comparing them to measurements from other well-established techniques. We imaged gold-coated HeLa cells to illustrate the tradeoffs between epi-wDPM with low and high spatial coherence.

Original languageEnglish (US)
Pages (from-to)6162-6165
Number of pages4
JournalOptics Letters
Volume39
Issue number21
DOIs
StatePublished - Jan 1 2014

Fingerprint

illumination
microscopy
tradeoffs
diffraction
shot
acquisition
microscopes
gold
geometry
cells

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics

Cite this

Epi-illumination diffraction phase microscopy with white light. / Edwards, C.; Bhaduri, B.; Griffin, B. G.; Goddard, L. L.; Popescu, G.

In: Optics Letters, Vol. 39, No. 21, 01.01.2014, p. 6162-6165.

Research output: Contribution to journalArticle

Edwards, C. ; Bhaduri, B. ; Griffin, B. G. ; Goddard, L. L. ; Popescu, G. / Epi-illumination diffraction phase microscopy with white light. In: Optics Letters. 2014 ; Vol. 39, No. 21. pp. 6162-6165.
@article{b86d369fb8254430953c80ec671a99cf,
title = "Epi-illumination diffraction phase microscopy with white light",
abstract = "We demonstrate the first reflection-based epi-illumination diffraction phase microscope with white light (epi-wDPM). The epi-wDPM system combines the off-axis, common-path, and white light approaches, in a reflection geometry enabling sub-nanometer spatial and temporal noise levels, while providing single-shot acquisition for opaque samples. We verified the epi-wDPM results by measuring control samples with known dimensions and comparing them to measurements from other well-established techniques. We imaged gold-coated HeLa cells to illustrate the tradeoffs between epi-wDPM with low and high spatial coherence.",
author = "C. Edwards and B. Bhaduri and Griffin, {B. G.} and Goddard, {L. L.} and G. Popescu",
year = "2014",
month = "1",
day = "1",
doi = "10.1364/OL.39.006162",
language = "English (US)",
volume = "39",
pages = "6162--6165",
journal = "Optics Letters",
issn = "0146-9592",
publisher = "The Optical Society",
number = "21",

}

TY - JOUR

T1 - Epi-illumination diffraction phase microscopy with white light

AU - Edwards, C.

AU - Bhaduri, B.

AU - Griffin, B. G.

AU - Goddard, L. L.

AU - Popescu, G.

PY - 2014/1/1

Y1 - 2014/1/1

N2 - We demonstrate the first reflection-based epi-illumination diffraction phase microscope with white light (epi-wDPM). The epi-wDPM system combines the off-axis, common-path, and white light approaches, in a reflection geometry enabling sub-nanometer spatial and temporal noise levels, while providing single-shot acquisition for opaque samples. We verified the epi-wDPM results by measuring control samples with known dimensions and comparing them to measurements from other well-established techniques. We imaged gold-coated HeLa cells to illustrate the tradeoffs between epi-wDPM with low and high spatial coherence.

AB - We demonstrate the first reflection-based epi-illumination diffraction phase microscope with white light (epi-wDPM). The epi-wDPM system combines the off-axis, common-path, and white light approaches, in a reflection geometry enabling sub-nanometer spatial and temporal noise levels, while providing single-shot acquisition for opaque samples. We verified the epi-wDPM results by measuring control samples with known dimensions and comparing them to measurements from other well-established techniques. We imaged gold-coated HeLa cells to illustrate the tradeoffs between epi-wDPM with low and high spatial coherence.

UR - http://www.scopus.com/inward/record.url?scp=84919782520&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84919782520&partnerID=8YFLogxK

U2 - 10.1364/OL.39.006162

DO - 10.1364/OL.39.006162

M3 - Article

AN - SCOPUS:84919782520

VL - 39

SP - 6162

EP - 6165

JO - Optics Letters

JF - Optics Letters

SN - 0146-9592

IS - 21

ER -