Enzyme treatments on corn fiber from wet-milling process for increased starch and protein extraction

Oguz K. Ozturk, Svend G. Kaasgaard, Lorena G. Palmén, Bernardo C. Vidal, Bruce R. Hamaker

Research output: Contribution to journalArticlepeer-review


Complete starch extraction has been a problem in the corn wet-milling, and here enzymes affecting different structures were used to provide a thorough understanding of the contribution of those structures to starch and protein retention for a way to achieve higher extraction rates. The combination of Frontia Fiberwash® (mix of cellulases and xylanases) and Olexa® (protease) led to an increase in starch (4.5 %) and protein (3.0 %) extraction. Enzymes changed secondary structure composition of proteins due to change in concentration of protein sub-fractions. The protein matrix around starch granules, the key factor for starch retention, was weakened with reducing agents/acids or ultrasound, which better separated starch and protein bodies. Chemicals led to 3.3–5.4 % and 0.7–1.7 % increase in starch and protein extraction, respectively, whereas an ultrasound treatment resulted in 2.2 % and 0.6 % increase. Overall, the study showed starch granules and protein bodies can be freed from wet-milled fiber fraction leading to higher extraction rate when the protein matrix is loosened.

Original languageEnglish (US)
Article number113622
JournalIndustrial Crops and Products
StatePublished - Sep 15 2021
Externally publishedYes


  • Corn fiber
  • Enzyme
  • Protein matrix
  • Reducing agent
  • Starch retention
  • Wet-milling

ASJC Scopus subject areas

  • Agronomy and Crop Science


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