Enzyme (re)design

lessons from natural evolution and computation

John Alan Gerlt, Patricia C. Babbitt

Research output: Contribution to journalReview article

Abstract

The (re)design of enzymes to catalyze 'new' reactions is a topic of considerable practical and intellectual interest. Directed evolution (random mutagenesis followed by screening/selection) has been used widely to identify novel biocatalysts. However, 'rational' approaches using either natural divergent evolution or computational predictions based on chemical principles have been less successful. This review summarizes recent progress in evolution-based and computation-based (re)design.

Original languageEnglish (US)
Pages (from-to)10-18
Number of pages9
JournalCurrent Opinion in Chemical Biology
Volume13
Issue number1
DOIs
StatePublished - Feb 1 2009

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Mutagenesis
Enzymes
Screening

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry

Cite this

Enzyme (re)design : lessons from natural evolution and computation. / Gerlt, John Alan; Babbitt, Patricia C.

In: Current Opinion in Chemical Biology, Vol. 13, No. 1, 01.02.2009, p. 10-18.

Research output: Contribution to journalReview article

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