Enzyme (re)design: lessons from natural evolution and computation

John A. Gerlt; Patricia C. Babbitt

doi:10.1016/j.cbpa.2009.01.014

Enzyme (re)design: lessons from natural evolution and computation

John A. Gerlt, Patricia C. Babbitt

Research output: Contribution to journal › Review article › peer-review

Abstract

The (re)design of enzymes to catalyze 'new' reactions is a topic of considerable practical and intellectual interest. Directed evolution (random mutagenesis followed by screening/selection) has been used widely to identify novel biocatalysts. However, 'rational' approaches using either natural divergent evolution or computational predictions based on chemical principles have been less successful. This review summarizes recent progress in evolution-based and computation-based (re)design.

Original language	English (US)
Pages (from-to)	10-18
Number of pages	9
Journal	Current Opinion in Chemical Biology
Volume	13
Issue number	1
DOIs	https://doi.org/10.1016/j.cbpa.2009.01.014
State	Published - Feb 2009

ASJC Scopus subject areas

Analytical Chemistry
Biochemistry

Online availability

10.1016/j.cbpa.2009.01.014

Library availability

Discover UIUC Full Text

Cite this

@article{d1bafcec9d444b97bf7b51c04787a2f2,

title = "Enzyme (re)design: lessons from natural evolution and computation",

abstract = "The (re)design of enzymes to catalyze 'new' reactions is a topic of considerable practical and intellectual interest. Directed evolution (random mutagenesis followed by screening/selection) has been used widely to identify novel biocatalysts. However, 'rational' approaches using either natural divergent evolution or computational predictions based on chemical principles have been less successful. This review summarizes recent progress in evolution-based and computation-based (re)design.",

author = "Gerlt, {John A.} and Babbitt, {Patricia C.}",

note = "Funding Information: The authors thank NIH R01 GM052594 (to JAG), R01 GM060595 (to PCB), R01 GM065155 (to JAG), and P01 GM071790 (to JAG and PCB) for support of research in their laboratories.",

year = "2009",

month = feb,

doi = "10.1016/j.cbpa.2009.01.014",

language = "English (US)",

volume = "13",

pages = "10--18",

journal = "Current Opinion in Chemical Biology",

issn = "1367-5931",

publisher = "Elsevier Ltd",

number = "1",

}

TY - JOUR

T1 - Enzyme (re)design

T2 - lessons from natural evolution and computation

AU - Gerlt, John A.

AU - Babbitt, Patricia C.

N1 - Funding Information: The authors thank NIH R01 GM052594 (to JAG), R01 GM060595 (to PCB), R01 GM065155 (to JAG), and P01 GM071790 (to JAG and PCB) for support of research in their laboratories.

PY - 2009/2

Y1 - 2009/2

N2 - The (re)design of enzymes to catalyze 'new' reactions is a topic of considerable practical and intellectual interest. Directed evolution (random mutagenesis followed by screening/selection) has been used widely to identify novel biocatalysts. However, 'rational' approaches using either natural divergent evolution or computational predictions based on chemical principles have been less successful. This review summarizes recent progress in evolution-based and computation-based (re)design.

AB - The (re)design of enzymes to catalyze 'new' reactions is a topic of considerable practical and intellectual interest. Directed evolution (random mutagenesis followed by screening/selection) has been used widely to identify novel biocatalysts. However, 'rational' approaches using either natural divergent evolution or computational predictions based on chemical principles have been less successful. This review summarizes recent progress in evolution-based and computation-based (re)design.

UR - http://www.scopus.com/inward/record.url?scp=65249143885&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=65249143885&partnerID=8YFLogxK

U2 - 10.1016/j.cbpa.2009.01.014

DO - 10.1016/j.cbpa.2009.01.014

M3 - Review article

C2 - 19237310

AN - SCOPUS:65249143885

SN - 1367-5931

VL - 13

SP - 10

EP - 18

JO - Current Opinion in Chemical Biology

JF - Current Opinion in Chemical Biology

IS - 1

ER -

Enzyme (re)design: lessons from natural evolution and computation

Abstract

ASJC Scopus subject areas

Online availability

Library availability

Related links

Fingerprint

Cite this