Enzyme (re)design: lessons from natural evolution and computation

John A. Gerlt, Patricia C. Babbitt

Research output: Contribution to journalReview articlepeer-review


The (re)design of enzymes to catalyze 'new' reactions is a topic of considerable practical and intellectual interest. Directed evolution (random mutagenesis followed by screening/selection) has been used widely to identify novel biocatalysts. However, 'rational' approaches using either natural divergent evolution or computational predictions based on chemical principles have been less successful. This review summarizes recent progress in evolution-based and computation-based (re)design.

Original languageEnglish (US)
Pages (from-to)10-18
Number of pages9
JournalCurrent Opinion in Chemical Biology
Issue number1
StatePublished - Feb 2009

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry


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