Enzyme-catalysed biodegradation of carbon dots follows sequential oxidation in a time dependent manner

Indrajit Srivastava, Dinabandhu Sar, Prabuddha Mukherjee, Aaron S. Schwartz-Duval, Zhaolu Huang, Camilo Jaramillo, Ana Civantos, Indu Tripathi, Jean Paul Allain, Rohit Bhargava, Dipanjan Pan

Research output: Contribution to journalArticle

Abstract

Carbon dots (CDs) have recently garnered significant attention owing to their excellent luminescence properties, thereby demonstrating a variety of applications in in vitro and in vivo imaging. Understanding the long-term metabolic fate of these agents in a biological environment is the focus of this work. Here we show that the CDs undergo peroxide catalysed degradation in the presence of lipase. Our results indicate that differently charged CD species exhibit unique degradation kinetics upon being subjected to enzyme oxidation. Furthermore, this decomposition correlates with the relative accessibility of the enzymatic molecule. Using multiple physico-chemical characterization studies and molecular modelling, we confirmed the interaction of passivating surface abundant molecules with the enzyme. Finally, we have identified hydroxymethyl furfural as a metabolic by-product of the CDs used here. Our results indicate the possibility and a likely mechanism for complete CD degradation in living systems that can pave the way for a variety of biomedical applications.

Original languageEnglish (US)
Pages (from-to)8226-8236
Number of pages11
JournalNanoscale
Volume11
Issue number17
DOIs
StatePublished - May 7 2019

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Biodegradation
Carbon
Enzymes
Oxidation
Degradation
Furaldehyde
Furfural
Enzyme kinetics
Molecules
Molecular modeling
Peroxides
Lipases
Lipase
Byproducts
Luminescence
Decomposition
Imaging techniques
Kinetics

ASJC Scopus subject areas

  • Materials Science(all)

Cite this

Srivastava, I., Sar, D., Mukherjee, P., Schwartz-Duval, A. S., Huang, Z., Jaramillo, C., ... Pan, D. (2019). Enzyme-catalysed biodegradation of carbon dots follows sequential oxidation in a time dependent manner. Nanoscale, 11(17), 8226-8236. https://doi.org/10.1039/c9nr00194h

Enzyme-catalysed biodegradation of carbon dots follows sequential oxidation in a time dependent manner. / Srivastava, Indrajit; Sar, Dinabandhu; Mukherjee, Prabuddha; Schwartz-Duval, Aaron S.; Huang, Zhaolu; Jaramillo, Camilo; Civantos, Ana; Tripathi, Indu; Allain, Jean Paul; Bhargava, Rohit; Pan, Dipanjan.

In: Nanoscale, Vol. 11, No. 17, 07.05.2019, p. 8226-8236.

Research output: Contribution to journalArticle

Srivastava, I, Sar, D, Mukherjee, P, Schwartz-Duval, AS, Huang, Z, Jaramillo, C, Civantos, A, Tripathi, I, Allain, JP, Bhargava, R & Pan, D 2019, 'Enzyme-catalysed biodegradation of carbon dots follows sequential oxidation in a time dependent manner', Nanoscale, vol. 11, no. 17, pp. 8226-8236. https://doi.org/10.1039/c9nr00194h
Srivastava I, Sar D, Mukherjee P, Schwartz-Duval AS, Huang Z, Jaramillo C et al. Enzyme-catalysed biodegradation of carbon dots follows sequential oxidation in a time dependent manner. Nanoscale. 2019 May 7;11(17):8226-8236. https://doi.org/10.1039/c9nr00194h
Srivastava, Indrajit ; Sar, Dinabandhu ; Mukherjee, Prabuddha ; Schwartz-Duval, Aaron S. ; Huang, Zhaolu ; Jaramillo, Camilo ; Civantos, Ana ; Tripathi, Indu ; Allain, Jean Paul ; Bhargava, Rohit ; Pan, Dipanjan. / Enzyme-catalysed biodegradation of carbon dots follows sequential oxidation in a time dependent manner. In: Nanoscale. 2019 ; Vol. 11, No. 17. pp. 8226-8236.
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