@article{9bc9155a93e546c8bb8d5d1933626f06,
title = "Enzymatic Synthesis of the Unnatural Nucleotide 2{\textquoteright}-Deoxyisoguanosine 5{\textquoteright}-Monophosphate",
abstract = "Naturally occurring DNA contains four canonical bases, forming two Watson-Crick base pairs (adenine-thymine, guanine-cytosine). Efforts over the past decades have led to the development of several unnatural base pairs, enabling the synthesis of unnatural DNA with an expanded genetic alphabet. The engineering of organisms capable of de novo biosynthesis of unnatural DNA would have significant technological and philosophical implications, but remains a challenge. Here we report the enzymatic conversion of 2{\textquoteright}-deoxyxanthosine 5{\textquoteright}-monophosphate (dXMP) into deoxyisoguanosine monophosphate (dBMP), a precursor of the unnatural isoguanine-isocytosine base pair. The reaction is catalyzed by the bacteriophage enzyme PurZ and bacterial PurB, and is a key addition to the toolbox for de novo biosynthesis of unnatural DNA.",
keywords = "DNA, adenylosuccinate synthetases, bacteriophages, isoguanine, unnatural base pairs",
author = "Fuping Zhao and Yifeng Wei and Xinyu Wang and Yan Zhou and Yang Tong and Ang, {Ee Lui} and Shengnan Liu and Huimin Zhao and Yan Zhang",
note = "We thank the instrument analytical center of School of Pharmaceutical Science and Technology at Tianjin University, in particular, Ms Yan Gao and Mr. Zhi Li for providing assistance in our LC–MS analysis. This work was supported by the NSFC Distinguished Young Scholar of China Program 32125002 (Y. Zhang), the Agency for Science, Technology and Research of Singapore Visiting Investigator Program Grant 1535j00137 (H.Z.), Advanced Manufacturing and Engineering Programmatic Grant A18A9b0060 (Y.W., E.L.A. and H.Z.) and the funding support from Agency for Science, Technology and Research C211917011 (Y.W., E.L.A. and H.Z.). We thank the instrument analytical center of School of Pharmaceutical Science and Technology at Tianjin University, in particular, Ms Yan Gao and Mr. Zhi Li for providing assistance in our LC–MS analysis. This work was supported by the NSFC Distinguished Young Scholar of China Program 32125002 (Y. Zhang), the Agency for Science, Technology and Research of Singapore Visiting Investigator Program Grant 1535j00137 (H.Z.), Advanced Manufacturing and Engineering Programmatic Grant A18A9b0060 (Y.W., E.L.A. and H.Z.) and the funding support from Agency for Science, Technology and Research C211917011 (Y.W., E.L.A. and H.Z.).",
year = "2022",
month = nov,
day = "4",
doi = "10.1002/cbic.202200295",
language = "English (US)",
volume = "23",
journal = "ChemBioChem",
issn = "1439-4227",
publisher = "John Wiley & Sons, Ltd.",
number = "21",
}