Enzymatic synthesis of β-[U-14C]alanine and d-[1,2,3-14C]pantothenate of high specific radioactivity

John E. Cronan

doi:10.1016/0003-2697(80)90626-0

Enzymatic synthesis of β-[U-¹⁴C]alanine and d-[1,2,3-¹⁴C]pantothenate of high specific radioactivity

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Abstract

β-[U-¹⁴C]Alanine can be synthesized in >95% yield from l-[U-¹⁴C]aspartic acid using the aspartate 1-decarboxylase of Escherichia coli and converted to d-[1,2,3-¹⁴C]pantothenate in a 10-20% yield using the pantothenate synthetase of E. coli. Sufficiently pure preparations of both enzymes are readily obtained.

Original language	English (US)
Pages (from-to)	377-380
Number of pages	4
Journal	Analytical Biochemistry
Volume	103
Issue number	2
DOIs	https://doi.org/10.1016/0003-2697(80)90626-0
State	Published - Apr 1980

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/0003-2697(80)90626-0

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Cite this

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abstract = "β-[U-14C]Alanine can be synthesized in >95% yield from l-[U-14C]aspartic acid using the aspartate 1-decarboxylase of Escherichia coli and converted to d-[1,2,3-14C]pantothenate in a 10-20% yield using the pantothenate synthetase of E. coli. Sufficiently pure preparations of both enzymes are readily obtained.",

author = "Cronan, {John E.}",

note = "Funding Information: by NIH Grant AI",

year = "1980",

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