Enzymatic and Chemical Structure Mapping of Mouse 28S Ribosomal Ribonucleic Acid Contacts in 5.8S Ribosomal Ribonucleic Acid

Thomas A. Walker, Kimberly D. Johnson, Gary J Olsen, Mary A. Peters, Norman R. Pace

Research output: Contribution to journalArticle

Abstract

Secondary structure mapping experiments using SI nuclease, RNase Th and diethyl pyrocarbonate as conformational probes have identified those regions in mouse 5.8S rRNA containing major sites of interaction with 28S rRNA. One site encompasses the 3'-terminal 20 nucleotides and corresponds to the region identified previously as a component of an RNase-resistant 5.8S/28S rRNA junction complex. A second site, located at the 5’ terminus, has not been defined precisely but is believed to involve approximately 20-30 nucleotides. The existence of these sites of interaction is supported by comparing sequences of eukaryotic 5.8S and 28S rRNA with those of the prokaryotic 23S rRNA. Evidence for the occurrence of at least three helical regions in the central portion of the mouse 5.8S rRNA molecule is also presented.

Original languageEnglish (US)
Pages (from-to)2320-2329
Number of pages10
JournalBiochemistry
Volume21
Issue number10
DOIs
StatePublished - May 1982
Externally publishedYes

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Ribonucleases
Nucleotides
Diethyl Pyrocarbonate
RNA
Molecules
Experiments

ASJC Scopus subject areas

  • Biochemistry

Cite this

Enzymatic and Chemical Structure Mapping of Mouse 28S Ribosomal Ribonucleic Acid Contacts in 5.8S Ribosomal Ribonucleic Acid. / Walker, Thomas A.; Johnson, Kimberly D.; Olsen, Gary J; Peters, Mary A.; Pace, Norman R.

In: Biochemistry, Vol. 21, No. 10, 05.1982, p. 2320-2329.

Research output: Contribution to journalArticle

Walker, Thomas A. ; Johnson, Kimberly D. ; Olsen, Gary J ; Peters, Mary A. ; Pace, Norman R. / Enzymatic and Chemical Structure Mapping of Mouse 28S Ribosomal Ribonucleic Acid Contacts in 5.8S Ribosomal Ribonucleic Acid. In: Biochemistry. 1982 ; Vol. 21, No. 10. pp. 2320-2329.
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