Enterolyin S, a Polythiazole-containing Hemolytic Peptide from Enterococcus caccae

Chengyou Shi, Varshal A. Patel, Douglas A. Mitchell, Huimin Zhao

Research output: Contribution to journalArticlepeer-review


The β-hemolytic factor streptolysin S (SLS) is an important linear azol(in)e-containing peptide (LAP) that contributes significantly to the virulence of Streptococcus pyogenes. Despite its discovery 85 years ago, SLS has evaded structural characterizing owing to its notoriously problematic physicochemical properties. Here, we report the discovery and characterization of a structurally analogous hemolytic peptide from Enterococcus caccae, termed enterolysin S (ELS). Through heterologous expression, site-directed mutagenesis, chemoselective modification, and high-resolution mass spectrometry, we found that ELS contains an intriguing contiguous octathiazole moiety. The discovery of ELS expands our knowledge of hemolytic LAPs by adding a new member to this virulence-promoting family of modified peptides.
Original languageEnglish (US)
Article numbere202400212
StateE-pub ahead of print - Apr 22 2024


  • cytolysin
  • post-translational modification
  • RiPPs
  • virulence factor
  • streptolysin S
  • YcaO
  • peptide
  • thiazole
  • LAP

ASJC Scopus subject areas

  • Molecular Medicine
  • Molecular Biology
  • Biochemistry
  • Organic Chemistry


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