Enrichment, cultivation, and detection of reductively dechlorinating bacteria

Frank E. Löffler, Robert A. Sanford, Kirsti M. Ritalahti

Research output: Contribution to journalReview articlepeer-review

Abstract

Strategies and procedures for enriching, isolating, and cultivating reductively dechlorinating bacteria that use chloroorganic compounds as metabolic electron acceptors from environmental samples are described. Further, nucleic acid-based approaches used to detect and quantify dechlorinator (i.e., Dehalococcoides)-specific genes are presented.

Original languageEnglish (US)
Pages (from-to)77-111
Number of pages35
JournalMethods in enzymology
Volume397
DOIs
StatePublished - 2005
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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