Enhancing DNA microarray detection limits using optical resonances from photonic crystal surfaces

P. C. Mathias, S. I. Jones, D. O. Gonzalez, L. O. Vodkin, B. T. Cunningham

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

DNA microarrays are typically composed of micronscale spots of specific genetic sequences attached to a glass microscope slide for the purpose of gene expression analysis. By replacing the glass microarray substrate with a photonic crystal surface, fluorescence enhancement effects can be utilized to improve the performance of the microarray. This work describes the design of photonic crystal surfaces by Rigorous Coupled-Wave Analysis to align guided-mode resonances with Cyanine-5 excitation and emission wavelengths at approximately 633 nm and 680 nm, respectively. The resonances from photonic crystals fabricated by nanoreplica molding are characterized by optical transmission measurements, and 20x enhancement from the fluorophore Cyanine-5 is demonstrated relative to a glass slide. Finally, a DNA microarray experiment probing the expression of genes in Glycine max (soybean) is performed, demonstrating the photonic crystal microarray detects 20% more genes from the sample than are detected by the glass microarray.

Original languageEnglish (US)
Title of host publicationTRANSDUCERS 2009 - 15th International Conference on Solid-State Sensors, Actuators and Microsystems
Pages730-733
Number of pages4
DOIs
StatePublished - Dec 11 2009
EventTRANSDUCERS 2009 - 15th International Conference on Solid-State Sensors, Actuators and Microsystems - Denver, CO, United States
Duration: Jun 21 2009Jun 25 2009

Publication series

NameTRANSDUCERS 2009 - 15th International Conference on Solid-State Sensors, Actuators and Microsystems

Other

OtherTRANSDUCERS 2009 - 15th International Conference on Solid-State Sensors, Actuators and Microsystems
CountryUnited States
CityDenver, CO
Period6/21/096/25/09

Keywords

  • DNA
  • Enhanced fluorescence
  • Guided-mode resonance
  • Microarray
  • Photonic crystal

ASJC Scopus subject areas

  • Hardware and Architecture
  • Electrical and Electronic Engineering

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    Mathias, P. C., Jones, S. I., Gonzalez, D. O., Vodkin, L. O., & Cunningham, B. T. (2009). Enhancing DNA microarray detection limits using optical resonances from photonic crystal surfaces. In TRANSDUCERS 2009 - 15th International Conference on Solid-State Sensors, Actuators and Microsystems (pp. 730-733). [5285438] (TRANSDUCERS 2009 - 15th International Conference on Solid-State Sensors, Actuators and Microsystems). https://doi.org/10.1109/SENSOR.2009.5285438