Enhanced susceptibility of photosynthesis to high leaf temperature in triazine-resistant Solanum nigrum L. Evidence for photosystem II D1 protein site of action

Jean Marc Ducruet, Donald Richard Ort

Research output: Contribution to journalArticle

Abstract

We have used nuclearly isogenic lines of Solanum nigrum, that differ genetically in the triazine-resistance trait, to investigate the mechanistic basis for the greater susceptibility of photosynthesis in resistant biotypes to elevated leaf temperature. Based on measurement of CO2-dependent oxygen evolution by leaf discs, of electron transfer partial reactions in isolated thylakoid membranes, and of chlorophyll fluorescence induction parameters of both leaves and thylakoid membranes, we conclude that: (1) The single base substitution in the chloroplast psb A gene that is responsible for triazine resistance is almost certainly also responsible for the decreased tolerance to high temperature; (2) The high temperature-induced inhibition of photosynthesis in the resistant biotype is caused by an inhibition of electron transfer between the primary and secondary quinone acceptors of photosystem II; (3) The enhanced temperature susceptibility is likely to be a significant contributing factor in the competitive disadvantage of triazine-resistant biotypes.

Original languageEnglish (US)
Pages (from-to)39-48
Number of pages10
JournalPlant Science
Volume56
Issue number1
DOIs
StatePublished - 1988

Fingerprint

Solanum nigrum
Triazines
D1 protein
Photosystem II Protein Complex
triazines
Photosynthesis
photosystem II
biotypes
photosynthesis
Thylakoids
Temperature
thylakoids
electron transfer
leaves
temperature
Proteins
Electrons
isogenic lines
Chloroplasts
Chlorophyll

Keywords

  • D protein
  • Solanum nigrum L
  • chlorophyll fluorescence
  • methyl purple
  • photosystem II
  • triazine-resistance

ASJC Scopus subject areas

  • Genetics
  • Agronomy and Crop Science
  • Plant Science

Cite this

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title = "Enhanced susceptibility of photosynthesis to high leaf temperature in triazine-resistant Solanum nigrum L. Evidence for photosystem II D1 protein site of action",
abstract = "We have used nuclearly isogenic lines of Solanum nigrum, that differ genetically in the triazine-resistance trait, to investigate the mechanistic basis for the greater susceptibility of photosynthesis in resistant biotypes to elevated leaf temperature. Based on measurement of CO2-dependent oxygen evolution by leaf discs, of electron transfer partial reactions in isolated thylakoid membranes, and of chlorophyll fluorescence induction parameters of both leaves and thylakoid membranes, we conclude that: (1) The single base substitution in the chloroplast psb A gene that is responsible for triazine resistance is almost certainly also responsible for the decreased tolerance to high temperature; (2) The high temperature-induced inhibition of photosynthesis in the resistant biotype is caused by an inhibition of electron transfer between the primary and secondary quinone acceptors of photosystem II; (3) The enhanced temperature susceptibility is likely to be a significant contributing factor in the competitive disadvantage of triazine-resistant biotypes.",
keywords = "D protein, Solanum nigrum L, chlorophyll fluorescence, methyl purple, photosystem II, triazine-resistance",
author = "Ducruet, {Jean Marc} and Ort, {Donald Richard}",
year = "1988",
doi = "10.1016/0168-9452(88)90183-5",
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journal = "Plant Science",
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TY - JOUR

T1 - Enhanced susceptibility of photosynthesis to high leaf temperature in triazine-resistant Solanum nigrum L. Evidence for photosystem II D1 protein site of action

AU - Ducruet, Jean Marc

AU - Ort, Donald Richard

PY - 1988

Y1 - 1988

N2 - We have used nuclearly isogenic lines of Solanum nigrum, that differ genetically in the triazine-resistance trait, to investigate the mechanistic basis for the greater susceptibility of photosynthesis in resistant biotypes to elevated leaf temperature. Based on measurement of CO2-dependent oxygen evolution by leaf discs, of electron transfer partial reactions in isolated thylakoid membranes, and of chlorophyll fluorescence induction parameters of both leaves and thylakoid membranes, we conclude that: (1) The single base substitution in the chloroplast psb A gene that is responsible for triazine resistance is almost certainly also responsible for the decreased tolerance to high temperature; (2) The high temperature-induced inhibition of photosynthesis in the resistant biotype is caused by an inhibition of electron transfer between the primary and secondary quinone acceptors of photosystem II; (3) The enhanced temperature susceptibility is likely to be a significant contributing factor in the competitive disadvantage of triazine-resistant biotypes.

AB - We have used nuclearly isogenic lines of Solanum nigrum, that differ genetically in the triazine-resistance trait, to investigate the mechanistic basis for the greater susceptibility of photosynthesis in resistant biotypes to elevated leaf temperature. Based on measurement of CO2-dependent oxygen evolution by leaf discs, of electron transfer partial reactions in isolated thylakoid membranes, and of chlorophyll fluorescence induction parameters of both leaves and thylakoid membranes, we conclude that: (1) The single base substitution in the chloroplast psb A gene that is responsible for triazine resistance is almost certainly also responsible for the decreased tolerance to high temperature; (2) The high temperature-induced inhibition of photosynthesis in the resistant biotype is caused by an inhibition of electron transfer between the primary and secondary quinone acceptors of photosystem II; (3) The enhanced temperature susceptibility is likely to be a significant contributing factor in the competitive disadvantage of triazine-resistant biotypes.

KW - D protein

KW - Solanum nigrum L

KW - chlorophyll fluorescence

KW - methyl purple

KW - photosystem II

KW - triazine-resistance

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