Enhanced separation and detection of serum bilirubin species by capillary electrophoresis using a mixed anionic surfactant-protein buffer system with laser-induced fluorescence detection

Nian Wu, Jonathan V. Sweedler, Mei Lin

Research output: Contribution to journalArticlepeer-review

Abstract

The four major bilirubin species in serum are separated by capillary electrophoresis and detected using laser-induced fluorescence detection. The optimum buffer system consists of 40 mM sodium dodecyl sulfate (SDS)-0.012 mM bovine serum albumin (BSA). The use of the SDS-BSA mixture in the mobile phase allows for the separation of four major bilirubin species at physiological pH with untreated capillaries. The results show that the use of BSA as a run buffer modifier in SDS solution improves separation efficiency and increases sample solubility via pH changes of the run buffer. The limits of detection for the bilirubin species using laser-induced fluorescence are between 30 and 150 nM, depending on the bilirubin species; not only is this approximately two orders of magnitude lower than with visible-light absorption methods, it allows the bilirubin species in normal sera to be quantitatively measured without sample pretreatment.

Original languageEnglish (US)
Pages (from-to)185-191
Number of pages7
JournalJournal of Chromatography B: Biomedical Sciences and Applications
Volume654
Issue number2
DOIs
StatePublished - Apr 1 1994

ASJC Scopus subject areas

  • Chemistry(all)

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