Enhanced resistance to early blight in transgenic tomato lines expressing heterologous plant defense genes

Scott C. Schaefer; Ksenija Gasic; Bruno Cammue; Willem Broekaert; Els J.M. Van Damme; Willy J. Peumans; Schuyler S. Korban

doi:10.1007/s00425-005-0026-x

Enhanced resistance to early blight in transgenic tomato lines expressing heterologous plant defense genes

Scott C. Schaefer
, Ksenija Gasic
, Bruno Cammue
, Willem Broekaert
, Els J.M. Van Damme
, Willy J. Peumans
, Schuyler S. Korban

Natural Resources and Environmental Sciences

Research output: Contribution to journal › Article › peer-review

Abstract

Genes coding for an iris ribosomal-inactivating protein (I-RIP), a maize β-glucanase (M-GLU), and a Mirabilis jalapa antimicrobial peptide (Mj-AMP1) were separately introduced into tomato (Lycopersicon esculentum cv. Sweet Chelsea) cotyledons via Agrobacterium tumefaciens-mediated transformation. Transgenic lines carrying each of the transgenes were confirmed for integration into the tomato genome using Southern blot hybridization. Transcription of I-RIP, M-GLU, and Mj-AMP1 genes in various transgenic lines was determined using Northern blot analysis. Plants of selected transgenic lines were inoculated with a 2-3×10⁴ conidial spores/ml suspension of the fungal pathogen Alternaria solani, the causal agent of tomato early blight. Compared to control (non-transformed) plants, two transgenic lines carrying either a M-GLU or Mj-AMP1 showed enhanced resistance to early blight disease. None of the four lines carrying the I-RIP transgene showed increased resistance to early blight.

Original language	English (US)
Pages (from-to)	858-866
Number of pages	9
Journal	Planta
Volume	222
Issue number	5
DOIs	https://doi.org/10.1007/s00425-005-0026-x
State	Published - Nov 2005

Keywords

Alternaria solani
Antimicrobial peptides
Disease resistance
Lycopersicon esculentum
Pathogenesis-related proteins

ASJC Scopus subject areas

Genetics
Plant Science

Online availability

10.1007/s00425-005-0026-x

Library availability

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title = "Enhanced resistance to early blight in transgenic tomato lines expressing heterologous plant defense genes",

abstract = "Genes coding for an iris ribosomal-inactivating protein (I-RIP), a maize β-glucanase (M-GLU), and a Mirabilis jalapa antimicrobial peptide (Mj-AMP1) were separately introduced into tomato (Lycopersicon esculentum cv. Sweet Chelsea) cotyledons via Agrobacterium tumefaciens-mediated transformation. Transgenic lines carrying each of the transgenes were confirmed for integration into the tomato genome using Southern blot hybridization. Transcription of I-RIP, M-GLU, and Mj-AMP1 genes in various transgenic lines was determined using Northern blot analysis. Plants of selected transgenic lines were inoculated with a 2-3×104 conidial spores/ml suspension of the fungal pathogen Alternaria solani, the causal agent of tomato early blight. Compared to control (non-transformed) plants, two transgenic lines carrying either a M-GLU or Mj-AMP1 showed enhanced resistance to early blight disease. None of the four lines carrying the I-RIP transgene showed increased resistance to early blight.",

keywords = "Alternaria solani, Antimicrobial peptides, Disease resistance, Lycopersicon esculentum, Pathogenesis-related proteins",

author = "Schaefer, \{Scott C.\} and Ksenija Gasic and Bruno Cammue and Willem Broekaert and \{Van Damme\}, \{Els J.M.\} and Peumans, \{Willy J.\} and Korban, \{Schuyler S.\}",

note = "Acknowledgements We wish to thank Syngeta for providing us with clone of Mj-AMP1 . We also thank Dr. Majid Foolad (Penn State) for his advice on preparing the inoculum. This work was supported by funds from the University of Illinois Office of Research Project no. 65–0325.",

year = "2005",

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doi = "10.1007/s00425-005-0026-x",

language = "English (US)",

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pages = "858--866",

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AU - Schaefer, Scott C.

AU - Gasic, Ksenija

AU - Cammue, Bruno

AU - Broekaert, Willem

AU - Van Damme, Els J.M.

AU - Peumans, Willy J.

AU - Korban, Schuyler S.

N1 - Acknowledgements We wish to thank Syngeta for providing us with clone of Mj-AMP1 . We also thank Dr. Majid Foolad (Penn State) for his advice on preparing the inoculum. This work was supported by funds from the University of Illinois Office of Research Project no. 65–0325.

PY - 2005/11

Y1 - 2005/11

N2 - Genes coding for an iris ribosomal-inactivating protein (I-RIP), a maize β-glucanase (M-GLU), and a Mirabilis jalapa antimicrobial peptide (Mj-AMP1) were separately introduced into tomato (Lycopersicon esculentum cv. Sweet Chelsea) cotyledons via Agrobacterium tumefaciens-mediated transformation. Transgenic lines carrying each of the transgenes were confirmed for integration into the tomato genome using Southern blot hybridization. Transcription of I-RIP, M-GLU, and Mj-AMP1 genes in various transgenic lines was determined using Northern blot analysis. Plants of selected transgenic lines were inoculated with a 2-3×104 conidial spores/ml suspension of the fungal pathogen Alternaria solani, the causal agent of tomato early blight. Compared to control (non-transformed) plants, two transgenic lines carrying either a M-GLU or Mj-AMP1 showed enhanced resistance to early blight disease. None of the four lines carrying the I-RIP transgene showed increased resistance to early blight.

AB - Genes coding for an iris ribosomal-inactivating protein (I-RIP), a maize β-glucanase (M-GLU), and a Mirabilis jalapa antimicrobial peptide (Mj-AMP1) were separately introduced into tomato (Lycopersicon esculentum cv. Sweet Chelsea) cotyledons via Agrobacterium tumefaciens-mediated transformation. Transgenic lines carrying each of the transgenes were confirmed for integration into the tomato genome using Southern blot hybridization. Transcription of I-RIP, M-GLU, and Mj-AMP1 genes in various transgenic lines was determined using Northern blot analysis. Plants of selected transgenic lines were inoculated with a 2-3×104 conidial spores/ml suspension of the fungal pathogen Alternaria solani, the causal agent of tomato early blight. Compared to control (non-transformed) plants, two transgenic lines carrying either a M-GLU or Mj-AMP1 showed enhanced resistance to early blight disease. None of the four lines carrying the I-RIP transgene showed increased resistance to early blight.

KW - Alternaria solani

KW - Antimicrobial peptides

KW - Disease resistance

KW - Lycopersicon esculentum

KW - Pathogenesis-related proteins

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ER -

Enhanced resistance to early blight in transgenic tomato lines expressing heterologous plant defense genes

Abstract

Keywords

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