Abstract
Genes coding for an iris ribosomal-inactivating protein (I-RIP), a maize β-glucanase (M-GLU), and a Mirabilis jalapa antimicrobial peptide (Mj-AMP1) were separately introduced into tomato (Lycopersicon esculentum cv. Sweet Chelsea) cotyledons via Agrobacterium tumefaciens-mediated transformation. Transgenic lines carrying each of the transgenes were confirmed for integration into the tomato genome using Southern blot hybridization. Transcription of I-RIP, M-GLU, and Mj-AMP1 genes in various transgenic lines was determined using Northern blot analysis. Plants of selected transgenic lines were inoculated with a 2-3×104 conidial spores/ml suspension of the fungal pathogen Alternaria solani, the causal agent of tomato early blight. Compared to control (non-transformed) plants, two transgenic lines carrying either a M-GLU or Mj-AMP1 showed enhanced resistance to early blight disease. None of the four lines carrying the I-RIP transgene showed increased resistance to early blight.
Original language | English (US) |
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Pages (from-to) | 858-866 |
Number of pages | 9 |
Journal | Planta |
Volume | 222 |
Issue number | 5 |
DOIs | |
State | Published - Nov 2005 |
Keywords
- Alternaria solani
- Antimicrobial peptides
- Disease resistance
- Lycopersicon esculentum
- Pathogenesis-related proteins
ASJC Scopus subject areas
- Genetics
- Plant Science