Enhanced mRNA FISH with compact quantum dots

Yang Liu, Phuong Le, Sung Jun Lim, Liang Ma, Suresh Sarkar, Zhiyuan Han, Stephen J. Murphy, Farhad Kosari, George Vasmatzis, John C. Cheville, Andrew M Smith

Research output: Contribution to journalArticle

Abstract

Fluorescence in situ hybridization (FISH) is the primary technology used to image and count mRNA in single cells, but applications of the technique are limited by photophysical shortcomings of organic dyes. Inorganic quantum dots (QDs) can overcome these problems but years of development have not yielded viable QD-FISH probes. Here we report that macromolecular size thresholds limit mRNA labeling in cells, and that a new generation of compact QDs produces accurate mRNA counts. Compared with dyes, compact QD probes provide exceptional photostability and more robust transcript quantification due to enhanced brightness. New spectrally engineered QDs also allow quantification of multiple distinct mRNA transcripts at the single-molecule level in individual cells. We expect that QD-FISH will particularly benefit high-resolution gene expression studies in three dimensional biological specimens for which quantification and multiplexing are major challenges.

Original languageEnglish (US)
Article number4461
JournalNature communications
Volume9
Issue number1
DOIs
StatePublished - Dec 1 2018

Fingerprint

Quantum Dots
Fluorescence In Situ Hybridization
Semiconductor quantum dots
Fluorescence
quantum dots
fluorescence
Messenger RNA
Coloring Agents
dyes
cells
probes
gene expression
multiplexing
Multiplexing
Gene expression
Labeling
marking
Luminance
brightness
Technology

ASJC Scopus subject areas

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

Cite this

Enhanced mRNA FISH with compact quantum dots. / Liu, Yang; Le, Phuong; Lim, Sung Jun; Ma, Liang; Sarkar, Suresh; Han, Zhiyuan; Murphy, Stephen J.; Kosari, Farhad; Vasmatzis, George; Cheville, John C.; Smith, Andrew M.

In: Nature communications, Vol. 9, No. 1, 4461, 01.12.2018.

Research output: Contribution to journalArticle

Liu, Y, Le, P, Lim, SJ, Ma, L, Sarkar, S, Han, Z, Murphy, SJ, Kosari, F, Vasmatzis, G, Cheville, JC & Smith, AM 2018, 'Enhanced mRNA FISH with compact quantum dots', Nature communications, vol. 9, no. 1, 4461. https://doi.org/10.1038/s41467-018-06740-x
Liu Y, Le P, Lim SJ, Ma L, Sarkar S, Han Z et al. Enhanced mRNA FISH with compact quantum dots. Nature communications. 2018 Dec 1;9(1). 4461. https://doi.org/10.1038/s41467-018-06740-x
Liu, Yang ; Le, Phuong ; Lim, Sung Jun ; Ma, Liang ; Sarkar, Suresh ; Han, Zhiyuan ; Murphy, Stephen J. ; Kosari, Farhad ; Vasmatzis, George ; Cheville, John C. ; Smith, Andrew M. / Enhanced mRNA FISH with compact quantum dots. In: Nature communications. 2018 ; Vol. 9, No. 1.
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