@article{5fa44a9398b94b2e99d2328c3bcc2449,
title = "Enhanced and tunable fluorescent quantum dots within a single crystal of protein",
abstract = "The design and synthesis of bio-nano hybrid materials can not only provide new materials with novel properties, but also advance our fundamental understanding of interactions between biomolecules and their abiotic counterparts. Here, we report a new approach to achieving such a goal by growing CdS quantum dots (QDs) within single crystals of lysozyme protein. This bio-nano hybrid emitted much stronger red fluorescence than its counterpart without the crystal, and such fluorescence properties could be either enhanced or suppressed by the addition of Ag(I) or Hg(II), respectively. The three-dimensional incorporation of CdS QDs within the lysozyme crystals was revealed by scanning transmission electron microscopy with electron tomography. More importantly, since our approach did not disrupt the crystalline nature of the lysozyme crystals, the metal and protein interactions were able to be studied by X-ray crystallography, thus providing insight into the role of Cd(II) in the CdS QDs formation. [Figure not available: see fulltext.]",
keywords = "functional bio-nanomaterials, protein single crystals, quantum dots, tomography, X-ray crystallography",
author = "Hui Wei and Stephen House and Jiangjiexing Wu and Jiong Zhang and Zidong Wang and Ying He and Gao, {Elizabeth J.} and Yigui Gao and Howard Robinson and Wei Li and Jianmin Zuo and Robertson, {Ian M.} and Yi Lu",
note = "Funding Information: This work was supported by the US National Science Foundation (Nos. CMMI 0749028 and DMR-0117792). The authors thank C. Lei and W. Swiech for help with the STEM imaging, C. M. Bee and D. Zhang for fluorescence microscopic measurements, S. M. Nie for the use of Nuance system and A. M. Smith for insightful discussions. S. H. and I. M. R. acknowledge support from the US Department of Energy (grant No. DE-FC36-05GO15064). STEM experiments were carried out in part in the Frederick Seitz Materials Research Laboratory Central Facilities, University of Illinois. X-ray crystallographic data for this study were measured at beamline X12C of the National Synchrotron Light Source, Brookhaven National Laboratory. Financial support comes principally from the Offices of Biological and Environmental Research and of Basic Energy Sciences of the US Department of Energy, and from the National Center for Research Resources (No. P41RR012408) and the National Institute of General Medical Sciences (No. P41GM103473) of the National Institutes of Health.",
year = "2013",
month = sep,
doi = "10.1007/s12274-013-0348-0",
language = "English (US)",
volume = "6",
pages = "627--634",
journal = "Nano Research",
issn = "1998-0124",
publisher = "Press of Tsinghua University",
number = "9",
}