Engineering Yarrowia lipolytica for production of medium-chain fatty acids and alcohols

Charles D. Rutter, Shuyan Zhang, Christopher V. Rao

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Lipids are naturally derived products that offer an attractive, renewable alternative to petroleum-based hydrocarbons. While naturally produced long-chain fatty acids can replace some petroleum analogs, medium-chain fatty acid would more closely match the desired physical and chemical properties of currently employed petroleum products. In this study we engineered Yarrowia lipolytica, an oleaginous yeast that naturally produces lipids at high titers, to produce medium-chain fatty acids. Five different acyl-ACP thioesterases with specificity for medium chain acyl-ACP molecules were expressed in Y. lipolytica, resulting in formation of either decanoic or octanoic acid. These novel fatty acid products were found to comprise up to 40% of the total cell lipids. Furthermore, the reduction in chain length resulted in a two-fold increase in specific lipid productivity in these engineered strains. The medium-chain fatty acids were found to be incorporated into all lipid classes.

Original languageEnglish (US)
Title of host publicationFood, Pharmaceutical and Bioengineering Division 2015 - Core Programming Area at the 2015 AIChE Meeting
PublisherAIChE
Pages40-48
Number of pages9
ISBN (Electronic)9781510818606
StatePublished - Jan 1 2015
EventFood, Pharmaceutical and Bioengineering Division 2015 - Core Programming Area at the 2015 AIChE Meeting - Salt Lake City, United States
Duration: Nov 8 2015Nov 13 2015

Publication series

NameFood, Pharmaceutical and Bioengineering Division 2015 - Core Programming Area at the 2015 AIChE Meeting
Volume1

Other

OtherFood, Pharmaceutical and Bioengineering Division 2015 - Core Programming Area at the 2015 AIChE Meeting
CountryUnited States
CitySalt Lake City
Period11/8/1511/13/15

ASJC Scopus subject areas

  • Engineering(all)
  • Food Science
  • Chemical Engineering(all)

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  • Cite this

    Rutter, C. D., Zhang, S., & Rao, C. V. (2015). Engineering Yarrowia lipolytica for production of medium-chain fatty acids and alcohols. In Food, Pharmaceutical and Bioengineering Division 2015 - Core Programming Area at the 2015 AIChE Meeting (pp. 40-48). (Food, Pharmaceutical and Bioengineering Division 2015 - Core Programming Area at the 2015 AIChE Meeting; Vol. 1). AIChE.