Engineering of NADPH regenerators in Escherichia coli for enhanced biotransformation

Won Heong Lee, Myoung Dong Kim, Yong Su Jin, Jin Ho Seo

Research output: Contribution to journalReview article

Abstract

Efficient regeneration of NADPH is one of the limiting factors that constrain the productivity of biotransformation processes. In order to increase the availability of NADPH for enhanced biotransformation by engineered Escherichia coli, modulation of the pentose phosphate pathway and amplification of the transhydrogenases system have been conventionally attempted as primary solutions. Recently, other approaches for stimulating NADPH regeneration during glycolysis, such as replacement of native glyceradehdye-3-phosphate dehydrogenase (GAPDH) with NADP-dependent GAPDH from Clostridium acetobutylicum and introduction of NADH kinase catalyzing direct phosphorylation of NADH to NADPH from Saccharomyces cerevisiae, were attempted and resulted in remarkable impacts on NADPH-dependent bioprocesses. This review summarizes several metabolic engineering approaches used for improving the NADPH regenerating capacity in engineered E. coli for whole-cell-based bioprocesses and discusses the key features and progress of those attempts.

Original languageEnglish (US)
Pages (from-to)2761-2772
Number of pages12
JournalApplied Microbiology and Biotechnology
Volume97
Issue number7
DOIs
StatePublished - Apr 1 2013

Keywords

  • Biotransformation process
  • Engineered Escherichia coli
  • NAD(H) kinase
  • NADP-dependent glyceradehdye-3- phosphate dehydrogenase
  • NADPH regeneration
  • Pentose phosphate pathway
  • Transhydrogenase

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

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