Engineering of NADPH regenerators in Escherichia coli for enhanced biotransformation

Won Heong Lee, Myoung Dong Kim, Yong-Su Jin, Jin Ho Seo

Research output: Contribution to journalReview article

Abstract

Efficient regeneration of NADPH is one of the limiting factors that constrain the productivity of biotransformation processes. In order to increase the availability of NADPH for enhanced biotransformation by engineered Escherichia coli, modulation of the pentose phosphate pathway and amplification of the transhydrogenases system have been conventionally attempted as primary solutions. Recently, other approaches for stimulating NADPH regeneration during glycolysis, such as replacement of native glyceradehdye-3-phosphate dehydrogenase (GAPDH) with NADP-dependent GAPDH from Clostridium acetobutylicum and introduction of NADH kinase catalyzing direct phosphorylation of NADH to NADPH from Saccharomyces cerevisiae, were attempted and resulted in remarkable impacts on NADPH-dependent bioprocesses. This review summarizes several metabolic engineering approaches used for improving the NADPH regenerating capacity in engineered E. coli for whole-cell-based bioprocesses and discusses the key features and progress of those attempts.

Original languageEnglish (US)
Pages (from-to)2761-2772
Number of pages12
JournalApplied Microbiology and Biotechnology
Volume97
Issue number7
DOIs
StatePublished - Apr 1 2013

Fingerprint

Biotransformation
NADP
Escherichia coli
NAD
Regeneration
Clostridium acetobutylicum
Phosphates
Metabolic Engineering
NADPH Dehydrogenase
Pentose Phosphate Pathway
Glycolysis
Saccharomyces cerevisiae
Oxidoreductases
Phosphotransferases
Phosphorylation

Keywords

  • Biotransformation process
  • Engineered Escherichia coli
  • NAD(H) kinase
  • NADP-dependent glyceradehdye-3- phosphate dehydrogenase
  • NADPH regeneration
  • Pentose phosphate pathway
  • Transhydrogenase

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

Cite this

Engineering of NADPH regenerators in Escherichia coli for enhanced biotransformation. / Lee, Won Heong; Kim, Myoung Dong; Jin, Yong-Su; Seo, Jin Ho.

In: Applied Microbiology and Biotechnology, Vol. 97, No. 7, 01.04.2013, p. 2761-2772.

Research output: Contribution to journalReview article

Lee, Won Heong ; Kim, Myoung Dong ; Jin, Yong-Su ; Seo, Jin Ho. / Engineering of NADPH regenerators in Escherichia coli for enhanced biotransformation. In: Applied Microbiology and Biotechnology. 2013 ; Vol. 97, No. 7. pp. 2761-2772.
@article{8b598b592b134fcd90ece1045724ccf3,
title = "Engineering of NADPH regenerators in Escherichia coli for enhanced biotransformation",
abstract = "Efficient regeneration of NADPH is one of the limiting factors that constrain the productivity of biotransformation processes. In order to increase the availability of NADPH for enhanced biotransformation by engineered Escherichia coli, modulation of the pentose phosphate pathway and amplification of the transhydrogenases system have been conventionally attempted as primary solutions. Recently, other approaches for stimulating NADPH regeneration during glycolysis, such as replacement of native glyceradehdye-3-phosphate dehydrogenase (GAPDH) with NADP-dependent GAPDH from Clostridium acetobutylicum and introduction of NADH kinase catalyzing direct phosphorylation of NADH to NADPH from Saccharomyces cerevisiae, were attempted and resulted in remarkable impacts on NADPH-dependent bioprocesses. This review summarizes several metabolic engineering approaches used for improving the NADPH regenerating capacity in engineered E. coli for whole-cell-based bioprocesses and discusses the key features and progress of those attempts.",
keywords = "Biotransformation process, Engineered Escherichia coli, NAD(H) kinase, NADP-dependent glyceradehdye-3- phosphate dehydrogenase, NADPH regeneration, Pentose phosphate pathway, Transhydrogenase",
author = "Lee, {Won Heong} and Kim, {Myoung Dong} and Yong-Su Jin and Seo, {Jin Ho}",
year = "2013",
month = "4",
day = "1",
doi = "10.1007/s00253-013-4750-z",
language = "English (US)",
volume = "97",
pages = "2761--2772",
journal = "Applied Microbiology and Biotechnology",
issn = "0175-7598",
publisher = "Springer Verlag",
number = "7",

}

TY - JOUR

T1 - Engineering of NADPH regenerators in Escherichia coli for enhanced biotransformation

AU - Lee, Won Heong

AU - Kim, Myoung Dong

AU - Jin, Yong-Su

AU - Seo, Jin Ho

PY - 2013/4/1

Y1 - 2013/4/1

N2 - Efficient regeneration of NADPH is one of the limiting factors that constrain the productivity of biotransformation processes. In order to increase the availability of NADPH for enhanced biotransformation by engineered Escherichia coli, modulation of the pentose phosphate pathway and amplification of the transhydrogenases system have been conventionally attempted as primary solutions. Recently, other approaches for stimulating NADPH regeneration during glycolysis, such as replacement of native glyceradehdye-3-phosphate dehydrogenase (GAPDH) with NADP-dependent GAPDH from Clostridium acetobutylicum and introduction of NADH kinase catalyzing direct phosphorylation of NADH to NADPH from Saccharomyces cerevisiae, were attempted and resulted in remarkable impacts on NADPH-dependent bioprocesses. This review summarizes several metabolic engineering approaches used for improving the NADPH regenerating capacity in engineered E. coli for whole-cell-based bioprocesses and discusses the key features and progress of those attempts.

AB - Efficient regeneration of NADPH is one of the limiting factors that constrain the productivity of biotransformation processes. In order to increase the availability of NADPH for enhanced biotransformation by engineered Escherichia coli, modulation of the pentose phosphate pathway and amplification of the transhydrogenases system have been conventionally attempted as primary solutions. Recently, other approaches for stimulating NADPH regeneration during glycolysis, such as replacement of native glyceradehdye-3-phosphate dehydrogenase (GAPDH) with NADP-dependent GAPDH from Clostridium acetobutylicum and introduction of NADH kinase catalyzing direct phosphorylation of NADH to NADPH from Saccharomyces cerevisiae, were attempted and resulted in remarkable impacts on NADPH-dependent bioprocesses. This review summarizes several metabolic engineering approaches used for improving the NADPH regenerating capacity in engineered E. coli for whole-cell-based bioprocesses and discusses the key features and progress of those attempts.

KW - Biotransformation process

KW - Engineered Escherichia coli

KW - NAD(H) kinase

KW - NADP-dependent glyceradehdye-3- phosphate dehydrogenase

KW - NADPH regeneration

KW - Pentose phosphate pathway

KW - Transhydrogenase

UR - http://www.scopus.com/inward/record.url?scp=84876674407&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84876674407&partnerID=8YFLogxK

U2 - 10.1007/s00253-013-4750-z

DO - 10.1007/s00253-013-4750-z

M3 - Review article

C2 - 23420268

AN - SCOPUS:84876674407

VL - 97

SP - 2761

EP - 2772

JO - Applied Microbiology and Biotechnology

JF - Applied Microbiology and Biotechnology

SN - 0175-7598

IS - 7

ER -