Abstract
Covering up to end 2015 Microbial fermentation provides an attractive alternative to chemical synthesis for the production of structurally complex natural products. In most cases, however, production titers are low and need to be improved for compound characterization and/or commercial production. Owing to advances in functional genomics and genetic engineering technologies, microbial hosts can be engineered to overproduce a desired natural product, greatly accelerating the traditionally time-consuming strain improvement process. This review covers recent developments and challenges in the engineering of native and heterologous microbial hosts for the production of bacterial natural products, focusing on the genetic tools and strategies for strain improvement. Special emphasis is placed on bioactive secondary metabolites from actinomycetes. The considerations for the choice of host systems will also be discussed in this review.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 963-987 |
| Number of pages | 25 |
| Journal | Natural Product Reports |
| Volume | 33 |
| Issue number | 8 |
| DOIs | |
| State | Published - Aug 2016 |
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ASJC Scopus subject areas
- Biochemistry
- Drug Discovery
- Organic Chemistry
Cite this
Engineering microbial hosts for production of bacterial natural products. / Zhang, Mingzi M.; Wang, Yajie; Ang, Ee Lui; Zhao, Huimin.
In: Natural Product Reports, Vol. 33, No. 8, 08.2016, p. 963-987.Research output: Contribution to journal › Review article
}
TY - JOUR
T1 - Engineering microbial hosts for production of bacterial natural products
AU - Zhang, Mingzi M.
AU - Wang, Yajie
AU - Ang, Ee Lui
AU - Zhao, Huimin
PY - 2016/8
Y1 - 2016/8
N2 - Covering up to end 2015 Microbial fermentation provides an attractive alternative to chemical synthesis for the production of structurally complex natural products. In most cases, however, production titers are low and need to be improved for compound characterization and/or commercial production. Owing to advances in functional genomics and genetic engineering technologies, microbial hosts can be engineered to overproduce a desired natural product, greatly accelerating the traditionally time-consuming strain improvement process. This review covers recent developments and challenges in the engineering of native and heterologous microbial hosts for the production of bacterial natural products, focusing on the genetic tools and strategies for strain improvement. Special emphasis is placed on bioactive secondary metabolites from actinomycetes. The considerations for the choice of host systems will also be discussed in this review.
AB - Covering up to end 2015 Microbial fermentation provides an attractive alternative to chemical synthesis for the production of structurally complex natural products. In most cases, however, production titers are low and need to be improved for compound characterization and/or commercial production. Owing to advances in functional genomics and genetic engineering technologies, microbial hosts can be engineered to overproduce a desired natural product, greatly accelerating the traditionally time-consuming strain improvement process. This review covers recent developments and challenges in the engineering of native and heterologous microbial hosts for the production of bacterial natural products, focusing on the genetic tools and strategies for strain improvement. Special emphasis is placed on bioactive secondary metabolites from actinomycetes. The considerations for the choice of host systems will also be discussed in this review.
UR - http://www.scopus.com/inward/record.url?scp=84979964893&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84979964893&partnerID=8YFLogxK
U2 - 10.1039/c6np00017g
DO - 10.1039/c6np00017g
M3 - Review article
C2 - 27072804
AN - SCOPUS:84979964893
VL - 33
SP - 963
EP - 987
JO - Natural Product Reports
JF - Natural Product Reports
SN - 0265-0568
IS - 8
ER -