Engineering microbial hosts for production of bacterial natural products

Mingzi M. Zhang; Yajie Wang; Ee Lui Ang; Huimin Zhao

doi:10.1039/c6np00017g

Engineering microbial hosts for production of bacterial natural products

Mingzi M. Zhang, Yajie Wang, Ee Lui Ang, Huimin Zhao

Research output: Contribution to journal › Review article › peer-review

Abstract

Covering up to end 2015 Microbial fermentation provides an attractive alternative to chemical synthesis for the production of structurally complex natural products. In most cases, however, production titers are low and need to be improved for compound characterization and/or commercial production. Owing to advances in functional genomics and genetic engineering technologies, microbial hosts can be engineered to overproduce a desired natural product, greatly accelerating the traditionally time-consuming strain improvement process. This review covers recent developments and challenges in the engineering of native and heterologous microbial hosts for the production of bacterial natural products, focusing on the genetic tools and strategies for strain improvement. Special emphasis is placed on bioactive secondary metabolites from actinomycetes. The considerations for the choice of host systems will also be discussed in this review.

Original language	English (US)
Pages (from-to)	963-987
Number of pages	25
Journal	Natural Product Reports
Volume	33
Issue number	8
DOIs	https://doi.org/10.1039/c6np00017g
State	Published - Aug 2016

ASJC Scopus subject areas

Biochemistry
Drug Discovery
Organic Chemistry

Access to Document

10.1039/c6np00017g

Fingerprint Dive into the research topics of 'Engineering microbial hosts for production of bacterial natural products'. Together they form a unique fingerprint.

Cite this

@article{2f702827997b424fa63150efe83d37c2,

title = "Engineering microbial hosts for production of bacterial natural products",

abstract = "Covering up to end 2015 Microbial fermentation provides an attractive alternative to chemical synthesis for the production of structurally complex natural products. In most cases, however, production titers are low and need to be improved for compound characterization and/or commercial production. Owing to advances in functional genomics and genetic engineering technologies, microbial hosts can be engineered to overproduce a desired natural product, greatly accelerating the traditionally time-consuming strain improvement process. This review covers recent developments and challenges in the engineering of native and heterologous microbial hosts for the production of bacterial natural products, focusing on the genetic tools and strategies for strain improvement. Special emphasis is placed on bioactive secondary metabolites from actinomycetes. The considerations for the choice of host systems will also be discussed in this review.",

author = "Zhang, {Mingzi M.} and Yajie Wang and Ang, {Ee Lui} and Huimin Zhao",

year = "2016",

month = aug,

doi = "10.1039/c6np00017g",

language = "English (US)",

volume = "33",

pages = "963--987",

journal = "Natural Product Reports",

issn = "0265-0568",

publisher = "Royal Society of Chemistry",

number = "8",

}

TY - JOUR

T1 - Engineering microbial hosts for production of bacterial natural products

AU - Zhang, Mingzi M.

AU - Wang, Yajie

AU - Ang, Ee Lui

AU - Zhao, Huimin

PY - 2016/8

Y1 - 2016/8

N2 - Covering up to end 2015 Microbial fermentation provides an attractive alternative to chemical synthesis for the production of structurally complex natural products. In most cases, however, production titers are low and need to be improved for compound characterization and/or commercial production. Owing to advances in functional genomics and genetic engineering technologies, microbial hosts can be engineered to overproduce a desired natural product, greatly accelerating the traditionally time-consuming strain improvement process. This review covers recent developments and challenges in the engineering of native and heterologous microbial hosts for the production of bacterial natural products, focusing on the genetic tools and strategies for strain improvement. Special emphasis is placed on bioactive secondary metabolites from actinomycetes. The considerations for the choice of host systems will also be discussed in this review.

AB - Covering up to end 2015 Microbial fermentation provides an attractive alternative to chemical synthesis for the production of structurally complex natural products. In most cases, however, production titers are low and need to be improved for compound characterization and/or commercial production. Owing to advances in functional genomics and genetic engineering technologies, microbial hosts can be engineered to overproduce a desired natural product, greatly accelerating the traditionally time-consuming strain improvement process. This review covers recent developments and challenges in the engineering of native and heterologous microbial hosts for the production of bacterial natural products, focusing on the genetic tools and strategies for strain improvement. Special emphasis is placed on bioactive secondary metabolites from actinomycetes. The considerations for the choice of host systems will also be discussed in this review.

UR - http://www.scopus.com/inward/record.url?scp=84979964893&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84979964893&partnerID=8YFLogxK

U2 - 10.1039/c6np00017g

DO - 10.1039/c6np00017g

M3 - Review article

C2 - 27072804

AN - SCOPUS:84979964893

VL - 33

SP - 963

EP - 987

JO - Natural Product Reports

JF - Natural Product Reports

SN - 0265-0568

IS - 8

ER -