Engineering and evolution of Yarrowia lipolytica for producing lipids from lignocellulosic hydrolysates

Sangdo Yook, Anshu Deewan, Leah Ziolkowski, Stephan Lane, Payman Tohidifar, Ming Hsun Cheng, Vijay Singh, Matthew J. Stasiewicz, Christopher V. Rao, Yong Su Jin

Research output: Contribution to journalArticlepeer-review

Abstract

Yarrowia lipolytica, an oleaginous yeast, shows promise for industrial fermentation due to its robust acetyl-CoA flux and well-developed genetic engineering tools. However, its lack of an active xylose metabolism restricts the conversion of cellulosic sugars to valuable products. To address this, metabolic engineering, and adaptive laboratory evolution (ALE) were applied to the Y. lipolytica PO1f strain, resulting in an efficient xylose-assimilating strain (XEV). Whole-genome sequencing (WGS) of the XEV followed by reverse engineering revealed that the amplification of the heterologous oxidoreductase pathway and a mutation in the GTPase-activating protein gene (YALI0B12100g) might be the primary reasons for improved xylose assimilation in the XEV strain. When a sorghum hydrolysate was used, the XEV strain showed superior xylose consumption and lipid production compared to its parental strain (X123). This study advances our understanding of xylose metabolism in Y. lipolytica and proposes effective metabolic engineering strategies for optimizing lignocellulosic hydrolysates.

Original languageEnglish (US)
Article number131806
JournalBioresource Technology
Volume416
DOIs
StatePublished - Jan 2025

Keywords

  • Adaptive laboratory evolution
  • Lignocellulosic hydrolysates
  • Reverse engineering
  • Whole genome sequencing
  • Yarrowia lipolytica

ASJC Scopus subject areas

  • Bioengineering
  • Environmental Engineering
  • Renewable Energy, Sustainability and the Environment
  • Waste Management and Disposal

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