Engineering a selective small-molecule substrate binding site into a deoxyribozyme

Claudia Höbartner, Scott K Silverman

Research output: Contribution to journalArticle

Abstract

A small goal: An RNA ligase deoxyribozyme is engineered to accept a small-molecule NTP substrate in a multiple-turnover fashion. Selective binding is enforced by hydrogen bonding, and structural preorganization within the NTP itself is important for its efficient utilization as a substrate. This study points the way toward a broader use of small-molecule substrates with nucleic acid enzymes. (Figure Presented)

Original languageEnglish (US)
Pages (from-to)7420-7424
Number of pages5
JournalAngewandte Chemie - International Edition
Volume46
Issue number39
DOIs
StatePublished - Oct 16 2007

Fingerprint

Catalytic DNA
Binding sites
Binding Sites
Molecules
Substrates
Nucleic acids
Ligases
RNA
Nucleic Acids
Hydrogen bonds
Enzymes

Keywords

  • Deoxyribozymes
  • Hydrogen bonds
  • Nucleic acids
  • RNA recognition
  • Receptors

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)

Cite this

Engineering a selective small-molecule substrate binding site into a deoxyribozyme. / Höbartner, Claudia; Silverman, Scott K.

In: Angewandte Chemie - International Edition, Vol. 46, No. 39, 16.10.2007, p. 7420-7424.

Research output: Contribution to journalArticle

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