Engineering a selective small-molecule substrate binding site into a deoxyribozyme

Claudia Höbartner; Scott K. Silverman

doi:10.1002/anie.200702217

Engineering a selective small-molecule substrate binding site into a deoxyribozyme

Claudia Höbartner, Scott K. Silverman

Chemistry

Research output: Contribution to journal › Article › peer-review

Abstract

A small goal: An RNA ligase deoxyribozyme is engineered to accept a small-molecule NTP substrate in a multiple-turnover fashion. Selective binding is enforced by hydrogen bonding, and structural preorganization within the NTP itself is important for its efficient utilization as a substrate. This study points the way toward a broader use of small-molecule substrates with nucleic acid enzymes. (Figure Presented)

Original language	English (US)
Pages (from-to)	7420-7424
Number of pages	5
Journal	Angewandte Chemie - International Edition
Volume	46
Issue number	39
DOIs	https://doi.org/10.1002/anie.200702217
State	Published - 2007

Keywords

Deoxyribozymes
Hydrogen bonds
Nucleic acids
RNA recognition
Receptors

ASJC Scopus subject areas

Catalysis
Chemistry(all)

Online availability

10.1002/anie.200702217

Library availability

Discover UIUC Full Text

Cite this

@article{146c18287dc54f66a418a77ae7ee8c40,

title = "Engineering a selective small-molecule substrate binding site into a deoxyribozyme",

abstract = "A small goal: An RNA ligase deoxyribozyme is engineered to accept a small-molecule NTP substrate in a multiple-turnover fashion. Selective binding is enforced by hydrogen bonding, and structural preorganization within the NTP itself is important for its efficient utilization as a substrate. This study points the way toward a broader use of small-molecule substrates with nucleic acid enzymes. (Figure Presented)",

keywords = "Deoxyribozymes, Hydrogen bonds, Nucleic acids, RNA recognition, Receptors",

author = "Claudia H{\"o}bartner and Silverman, {Scott K.}",

note = "Funding Information: The author is grateful to his supervisor Professor Kengo Hirachi for introducing him to this subject and for helpful comments. This work was supported by the Program for Leading Graduate Schools, MEXT, Japan.",

year = "2007",

doi = "10.1002/anie.200702217",

language = "English (US)",

volume = "46",

pages = "7420--7424",

journal = "Angewandte Chemie - International Edition",

issn = "1433-7851",

publisher = "John Wiley & Sons, Ltd.",

number = "39",

}

TY - JOUR

T1 - Engineering a selective small-molecule substrate binding site into a deoxyribozyme

AU - Höbartner, Claudia

AU - Silverman, Scott K.

N1 - Funding Information: The author is grateful to his supervisor Professor Kengo Hirachi for introducing him to this subject and for helpful comments. This work was supported by the Program for Leading Graduate Schools, MEXT, Japan.

PY - 2007

Y1 - 2007

N2 - A small goal: An RNA ligase deoxyribozyme is engineered to accept a small-molecule NTP substrate in a multiple-turnover fashion. Selective binding is enforced by hydrogen bonding, and structural preorganization within the NTP itself is important for its efficient utilization as a substrate. This study points the way toward a broader use of small-molecule substrates with nucleic acid enzymes. (Figure Presented)

AB - A small goal: An RNA ligase deoxyribozyme is engineered to accept a small-molecule NTP substrate in a multiple-turnover fashion. Selective binding is enforced by hydrogen bonding, and structural preorganization within the NTP itself is important for its efficient utilization as a substrate. This study points the way toward a broader use of small-molecule substrates with nucleic acid enzymes. (Figure Presented)

KW - Deoxyribozymes

KW - Hydrogen bonds

KW - Nucleic acids

KW - RNA recognition

KW - Receptors

UR - http://www.scopus.com/inward/record.url?scp=35048862889&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=35048862889&partnerID=8YFLogxK

U2 - 10.1002/anie.200702217

DO - 10.1002/anie.200702217

M3 - Article

C2 - 17694519

AN - SCOPUS:35048862889

SN - 1433-7851

VL - 46

SP - 7420

EP - 7424

JO - Angewandte Chemie - International Edition

JF - Angewandte Chemie - International Edition

IS - 39

ER -

Engineering a selective small-molecule substrate binding site into a deoxyribozyme

Abstract

Keywords

ASJC Scopus subject areas

Online availability

Library availability

Related links

Fingerprint

Cite this