Engineering a β-sheet protein toward the folding speed limit

Houbi Nguyen, Marcus Jäger, Jeffery W. Kelly, Martin Gruebele

Research output: Contribution to journalArticlepeer-review


Recent experimental studies have shown that α-helical proteins can approach the folding "speed limit", where folding switches from an activated to a downhill process in free energy. β-sheet proteins are generally thought to fold more slowly than helix bundles. However, based on studies of hairpins, folding should still be able to approach the microsecond time scale. Here we demonstrate how the hPinl WW domain, a triple-stranded β-sheet protein with a sharp thermodynamic melting transition, can be engineered toward the folding "speed limit" without a significant loss in thermal denaturation cooperativity.

Original languageEnglish (US)
Pages (from-to)15182-15186
Number of pages5
JournalJournal of Physical Chemistry B
Issue number32
StatePublished - Aug 18 2005

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Surfaces, Coatings and Films
  • Materials Chemistry


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