Engineered recombinant escherichia coli probiotic strains integrated with f4 and f18 fimbriae cluster genes in the chromosome and their assessment of immunogenic efficacy in vivo

Bingming Ou, Boyu Jiang, Duo Jin, Ying Yang, Minyu Zhang, Dong Zhang, Haizhou Zhao, Mengxian Xu, Haoliang Song, Wenwen Wu, Mingliang Chen, Ti Lu, Jiachen Huang, Hyesuk Seo, Carolina Garcia, Wanglong Zheng, Weiyi Guo, Yinhua Lu, Yu Jiang, Sheng YangRadhey S. Kaushik, Xinchang Li, Weiping Zhang, Guoqiang Zhu

Research output: Contribution to journalArticle

Abstract

F4 (K88) and F18 fimbriaed enterotoxigenic Escherichia coli (ETEC) are the predominant causes of porcine postweaning diarrhea (PWD), and vaccines are considered the most effective preventive approach against PWD. Since heterologous DNA integrated into bacterial chromosomes could be effectively expressed with stable inheritance, we chose probiotic EcNc (E. coli Nissle 1917 prototype cured of cryptic plasmids) as a delivery vector to express the heterologous F4 or both F4 and F18 fimbriae and sequentially assessed their immune efficacy of anti-F4 and F18 fimbriae in both murine and piglet models. Employing the CRISPR-cas9 technology, yjcS, pcadA, lacZ, yieN/trkD, maeB, and nth/tppB sites in the chromosome of an EcNc strain were targeted as integration sites to integrate F4 or F18 fimbriae cluster genes under the Ptet promotor to construct two recombinant integration probiotic strains (RIPSs), i.e., nth integration strain (EcNcΔnth/tppB::PtetF4) and multiple integration strain (EcNc::PtetF18x4::PtetF4x2). Expression of F4, both F4 and F18 fimbriae on the surfaces of two RIPSs, was verified with combined methods of agglutination assay, Western blot, and immunofluorescence microscopy. The recombinant strains have improved adherence to porcine intestinal epithelial cell lines. Mice and piglets immunized with the nth integration strain and multiple integration strain through gavage developed anti-F4 and both anti-F4 and anti-F18 IgG immune responses. Moreover, the serum antibodies from the immunized mice and piglets significantly inhibited the adherence of F4+ or both F4+ and F18+ ETEC wild-type strains to porcine intestinal cell lines in vitro, indicating the potential of RIPSs as promising probiotic strains plus vaccine candidates against F4+/F18+ ETEC infection.

Original languageEnglish (US)
Pages (from-to)412-426
Number of pages15
JournalACS synthetic biology
Volume9
Issue number2
DOIs
StatePublished - Feb 21 2020

Keywords

  • CRISPR/cas9
  • E. coli nissle 1917
  • Engineered recombinant probiotic strains
  • F18 fimbriae
  • F4(K88) fimbriae
  • Genomic integration

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biochemistry, Genetics and Molecular Biology (miscellaneous)

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  • Cite this

    Ou, B., Jiang, B., Jin, D., Yang, Y., Zhang, M., Zhang, D., Zhao, H., Xu, M., Song, H., Wu, W., Chen, M., Lu, T., Huang, J., Seo, H., Garcia, C., Zheng, W., Guo, W., Lu, Y., Jiang, Y., ... Zhu, G. (2020). Engineered recombinant escherichia coli probiotic strains integrated with f4 and f18 fimbriae cluster genes in the chromosome and their assessment of immunogenic efficacy in vivo. ACS synthetic biology, 9(2), 412-426. https://doi.org/10.1021/acssynbio.9b00430