Endopolyploidy in diploid and tetraploid maize (Zea mays l.)

D. P. Biradar, A. Lane Rayburn, D. G. Bullock

Research output: Contribution to journalArticle

Abstract

Nuclei from different tissues such as stem, mesocotyl, nodal root and root tip of diploid and tetraploid maize were isolated, stained with propidium iodide and passed through an EPICS-751 flow-cytometer cell sorter. Variations in flow histograms were observed in different tissues. Stem tissues of both the diploid and tetraploid had two peaks representing G1 and G2 somatic nuclei. The remaining tissues in both the diploids and tetraploids exhibited three peaks. The first peak observed in these tissues represents G1 somatic nuclei of the lowest ploidy level. The second peak represents G2 somatic nuclei of the lowest ploidy level -j- G1 somatic nuclei of the next ploidy level. The third peak represents G2 of the higher ploidy level + GI somatic nuclei of the next higher ploidy level. Statistically significant differences were observed between the diploid and tetraploid maize tissues with respect to nuclei distribution in the higher ploidy level peaks implying variation in the degree of endopolyploidy in the diploid and tetraploid maize. The results of this study suggest that the amount of endopolyploidy observed in maize genotypes has an effect on their overall agronomic performance under the field conditions.

Original languageEnglish (US)
Pages (from-to)417-421
Number of pages5
JournalAnnals of botany
Volume71
Issue number5
DOIs
StatePublished - May 1993

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endopolyploidy
ploidy
tetraploidy
diploidy
Zea mays
corn
stems
root tips
agronomic traits
tissues
genotype

Keywords

  • Diploid
  • Endopolyploidy
  • Flow cytometry
  • Maize
  • Tetraploid
  • Zea mays L

ASJC Scopus subject areas

  • Plant Science

Cite this

Endopolyploidy in diploid and tetraploid maize (Zea mays l.). / Biradar, D. P.; Lane Rayburn, A.; Bullock, D. G.

In: Annals of botany, Vol. 71, No. 5, 05.1993, p. 417-421.

Research output: Contribution to journalArticle

Biradar, D. P. ; Lane Rayburn, A. ; Bullock, D. G. / Endopolyploidy in diploid and tetraploid maize (Zea mays l.). In: Annals of botany. 1993 ; Vol. 71, No. 5. pp. 417-421.
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